特产研究2024,Vol.46Issue(1) :37-44,49.DOI:10.16720/j.cnki.tcyj.2024.012

毛酸浆多糖泡腾颗粒的制备及体外抗氧化活性研究

Study on the Preparation and Antioxidant Activity in Vitro of Polysaccha-ride Effervescent Granules from Physalis pubescens

李春梅 张馨元 刘爽 史彤辉 周长胜
特产研究2024,Vol.46Issue(1) :37-44,49.DOI:10.16720/j.cnki.tcyj.2024.012

毛酸浆多糖泡腾颗粒的制备及体外抗氧化活性研究

Study on the Preparation and Antioxidant Activity in Vitro of Polysaccha-ride Effervescent Granules from Physalis pubescens

李春梅 1张馨元 1刘爽 1史彤辉 1周长胜2
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作者信息

  • 1. 哈尔滨商业大学药学院,黑龙江 哈尔滨 150076
  • 2. 哈尔滨商业大学马克思主义学院,黑龙江 哈尔滨 150028
  • 折叠

摘要

为制备毛酸浆多糖泡腾颗粒剂并测定其抗氧化能力,本研究采取响应面法,筛选出泡腾颗粒剂的最佳处方,并对其进行质量评价.采用苯酚-浓硫酸检测法测定制剂中多糖的含量,并对其抗氧化能力进行测定.结果显示,最佳处方为15%的毛酸浆多糖粉末,碳酸氢钠与柠檬酸的质量比为1∶1.1,聚乙二醇6000与碳酸氢钠的质量比为0.3∶1,稀释剂为质量占比30%的甘露醇,黏合剂为6.4%的PVP-K30乙醇溶液.毛酸浆多糖泡腾颗粒有一定的还原力,其质量浓度与还原力呈正相关;在浓度为1.0mg/mL时,对·OH的清除率达到85.66%;在浓度为4.0 mg/mL时,对DPPH自由基的清除率达到83.45%;在浓度为4.0 mg/mL时,对超氧阴离子的清除率达到25.88%.毛酸浆多糖泡腾颗粒制剂服用方便、口味适宜,且具有良好的抗氧化活性.

Abstract

The aim of this study is to prepare polysaccharides effervescent granules of Physalis pubescens and determine its antioxidant ca-pacity.Response surface methodology was used to screen the best prescription of effervescent granules and evaluate its quality.The content of polysaccharide in the preparation was determined by phenol-concentrated sulfuric acid detection method.The antioxidant capacity was also measured.The results showed that the best prescription was 15%polysaccharide powder of pilose acid pulp,the mass ratio of sodium bicarbonate to citric acid was 1∶1.1,the mass ratio of polyethylene glycol 6000 to sodium bicarbonate was 0.3∶1,the diluent was mannitol with a mass ratio of 30%,the adhesive was PVP-K30 ethanol solution with a mass ratio of 6.4%.The polysaccharide effervescent granules of Physalis pubescens have certain reducing power,and its mass concentration is positively correlated with the reducing power;when the concentration is 1.0 mg/mL,the removal rate of·OH reaches 85.66%;when the concentration is 4.0 mg/mL,the scavenging rate of DPPH free radical reaches 83.45%;when the concentration is 4.0 mg/mL,the removal rate of superoxide anion reaches 25.88%.The polysaccharide effervescent granules of Physalis pubescens is convenient to take,suitable in taste,and has good antioxidant activity.

关键词

毛酸浆/多糖类/泡腾颗粒剂/抗氧化

Key words

Physalis pubescens/polysaccharides/effervescent granules/anti-oxidation

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基金项目

黑龙江省教育科学"十四五"规划省专项重点课题(GJE1422088)

2023年度黑龙江省中医药经典普及化专项课题(ZYW2023-077)

黑龙江省博士后科研启动金项目(BS0051)

哈尔滨商业大学博士科研启动项目(2019DS114)

出版年

2024
特产研究
中国农业科学院特产研究所,中国农学会特产学会

特产研究

CSTPCD
影响因子:0.331
ISSN:1001-4721
参考文献量18
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