The Protective Effect and Mechanism of Tribulus terrestris L.Fruit Extract on Ischemic Stroke Rats Based on UPLC-Q-Exactive-MS Metabolomics
To investigate the protection effect of Tribulus terrestris L.fruit extract on the middle cerebral artery occlusion(MCAO)-induced IS in rats based on the LC-MS metabolomics technique.30 rats were divided into the sham group randomly,the model group and adminis-tration group.The anti-IS effect of T terrestris fruit extract was conducted by the evaluation of neurological defects and cerebral infarct vol-ume percentage in rats.Based on the LC-MS untargeted metabolomics approach was used to conduct on rat urine to obtain the differential metabolites of T terrestris fruit extract anti-IS and analyze their metabolic pathways.T terrestris fruit extract treatment significantly lowered neurological deficit scores and cerebral infarct volume percentage in MCAO rats.By analyzing the urine metabolic profiles of model group and sham group,20 kinds of the differential metabolites were screened and identified,mainly involving three metabolic pathways.T terre-stris fruit extract is mainly through regulating the metabolism of valine,leucine and isoleucine biosynthesis,citrate cycle(TCA cycle)and folate biosynthesis to protect IS rats,so as to provide a theoretical basis for the rational clinical application.
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