Identification and VP2 Gene Sequence Analysis of Canine Parvovirus CPV-2c Asian Strain Isolation
Absrtact:To investigate the unknown death of dogs in Beijing kennel.A strain of CPV was isolated from the anal swabs of dogs which suf-fering from intestinal bleeding in a Beijing dog farm.The strain was identified by PCR,hemagglutination and indirect immunofluorescence.The VP2 gene were amplified and cloned by PCR,determined its genetic branch by sequence analyse.The results showed that the isolate strain belonged to Canine parvovirus type 2c and was named CPV-BJ21 strain.The indirect immunofluorescence results of the canine mono-clonal antibody were positive.Gene sequence analysis showed that CPV-BJ21 and CPV were the same evolutionary branch,the nucleotide sequence of VP2 gene had 99％homology with cpv-2c(MH476581)in Sichuan,China;CPV-BJ21 with close genetic relationship with Asian strains.There were amino acid mutations at A5G,S297A and D426E in VP2 amino acid sequence.After 3 generations of superculture of F81 cells,the hemagglutination titer of virus solution was stable at 210.This study could provide a reference for the prevalence of CPV and the development of new vaccines.
canine parvovirusisolation and identificationVP2 genephylogenetic analysis
国家科技期刊平台
NSTL
万方数据
