蜜蜂资源形态测定的采样与保存方法研究
Study on Sampling and Preservation Methods for Morphological Identification of Honeybee Resources
庄明亮 1李剑飞 1迟永娟 1葛蓬 1王志 1蒋海宾 1王进州 1戚竞文 1李志勇1
作者信息
- 1. 吉林省养蜂科学研究所,吉林 吉林 132108
- 折叠
摘要
形态测定是蜜蜂种质资源评价必不可少的技术手段,本研究旨在优化采样与保存方法来提高蜜蜂形态测定工作的效率.通过各试验组不同采样与保存方法对比蜜蜂样品的伸吻率、组织损坏数量、色度及韧性的变化.结果表明,试验组B毙杀20s、30s的伸吻率差异不显著(P＞0.05),但均显著高于其他试验组(P＜0.05);试验组D的蜜蜂组织损坏数量显著低于试验组E、F、G和对照组(P＜0.05);在4 ℃环境中保存1～3个月对蜜蜂样本的色度和样本的韧性均没有影响.蜜蜂资源形态测定的样品建议使用2L/min流速的CO2毙杀20 s,浸泡在75％酒精和0.5％甘油的保存液中,保存3个月内完成形态测定工作.
Abstract
In order to improve the efficiency of bee morphological identification,morphological identification is an essential technique for bee germplasm evaluation.In this study,different sampling and preservation methods were used to compare the kissing rate,the amount of tissue damage,the influence of chroma and the influence of toughness of bee samples in different experimental groups.The results showed that there was no significant difference in the kissing rate of B fumigation at 20 s and 30 s in experimental groups(P＞0.05),but it was sig-nificantly higher than that in other experimental groups(P＜0.05).The number of honeybee tissue damage in experimental group D was significantly lower than that in experimental group E,F,G and control group(P＜0.05).Storage at 4 ℃ for 1 to 3 months had no effect on the chroma and toughness of honeybee sampls.It is recommended to use CO2 at the flow rate of 2 L/min for 20 s,soak in the preservation solution of 75％alcohol and 0.5％glycerol,and complete the morphological identification within 3 months.
关键词
蜜蜂/采样/保存/资源鉴定/形态测定Key words
bees/sampling/preservation/morphological identification/morphological determination引用本文复制引用
基金项目
国家重点研发计划(2022YFD1600200)
吉林省科技发展计划(YDZJ202301ZYTS332)
国家蜂产业技术体系建设项目(CARS-44-KXJ3)
出版年
2024
国家科技期刊平台
NSTL
万方数据