Optimization of Extraction Process and Pharmacodynamic Evaluation of Tongshu Gel Plaster
To optimize the extraction process of four herbs in Tongshu gel plaster and to evaluate the pharmacodynamics.The effects of eth-anol concentration,solvent multiplication and extraction time on the extraction process of Tongshu gel plaster were investigated by using L9(34)3-factor 4-level orthogonal design test method,with the contents of tetrahydropalmatine,paeoniflorin,tetrandrine,and aconitine lac-tone and the extraction dry paste rate as the evaluation criteria.Rat model of cold-congealed blood stasis type dysmenorrhea was established,and rats were divided into four groups:control group,model group,dysmenorrhea ointment group,and Tongshu gel plaster group.The ef-fectiveness of Tongshu gel plaster in treating dysmenorrhea was evaluated by conducting the torsion reaction test in dysmenorrheic rats and using the enzyme-linked immunoassay was to determine the prostaglandin 2α(PGF2α)and prostaglandin(PGE2)in the uterine tissues of rats,and the content of estrogen(E2)and β-endorphin(β-EP)in serum to evaluate the efficacy of Tongshu gel plaster in the treatment of dys-menorrhea.The optimal extraction process of Tongshu gel plaster was as follows:add 8 times the amount of 80％concentration of ethanol in the herbs and soak for 30 min,heat and reflux the extract twice,each time for 2 h.The combined extracts were concentrated under reduced pressure to the appropriate amount and dried under reduced pressure in a vacuum drying oven.The Tongshu gel plaster prepared by the op-timal extraction process parameters could significantly reduce the number of twists and prolong the latency time of twists in dysmenorrheic rats(P＜0.01).Under the effect of Tongshu gel plaster,the content of PGF2α in the uterine tissues of rats decreased and the content of PGE2 increased(P＜0.01),and the content of E2 in serum decreased(P＜0.01)and the content of β-EP increased(P＜0.05).The preferred ex-traction process has high extraction efficiency,good stability and reproducibility and is simple and feasible.This study shows that,Tongshu gel plaster has good ameliorative effect on dysmenorrhea with cold clotting and blood stasis and analgesic efficacy.
Tongshu gel plasterTongshu recipeextraction processdysmenorrheapharmacodynamic
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