痛舒方凝胶膏剂的提取工艺优化及药效学评价
Optimization of Extraction Process and Pharmacodynamic Evaluation of Tongshu Gel Plaster
柳嘉 1杨雪 1刘婧 1关枫 1王佳婧 2王艳宏1
作者信息
- 1. 黑龙江中医药大学,黑龙江 哈尔滨 150040
- 2. 黑龙江中医药大学附属第二医院,黑龙江 哈尔滨 150001
- 折叠
摘要
本文旨在优选痛舒方凝胶膏剂中4味药材的提取工艺,并进行药效学评价.采用L9(34)3因素4水平正交设计试验法,以延胡索乙素、乌药醚内酯、芍药苷、粉防己碱含量以及提取干膏率为评价标准,考察乙醇浓度、溶剂倍量、提取时间对痛舒方凝胶膏剂提取工艺的影响.以寒凝血瘀型痛经大鼠为模型,设置空白组、模型组、痛经软膏组[为阳性对照,0.5g/(kg·d),3cm×3cm]、痛舒方凝胶膏剂组[含生药量13.65 g/(kg·d),3 cm×3 cm].通过进行痛经大鼠扭体反应试验和利用酶联免疫法,测定大鼠子宫组织前列腺素2α(PGF2α)、前列腺素(PGE2),血清中雌激素(E2)和β-内啡肽(β-EP)含量,评价痛舒方凝胶膏剂治疗痛经的疗效.结果表明,痛舒方凝胶膏剂的最佳提取工艺为:加入8倍量,80％浓度乙醇,加热回流提取2次,每次2 h.最佳提取工艺参数制备的痛舒方凝胶膏剂,能减少痛经大鼠扭体数、延长扭体潜伏时间(P＜0.01).在痛舒方凝胶膏剂作用下,大鼠子宫组织中PGF2α含量降低,PGE2含量升高(P＜0.01),血清中E2含量降低(P＜0.01),β-EP含量升高(P＜0.05).优选的提取工艺提取效率高,稳定性和重复性好且简单可行.本研究表明,痛舒方凝胶膏剂对于寒凝血瘀症痛经有很好的改善作用,有镇痛的疗效.
Abstract
To optimize the extraction process of four herbs in Tongshu gel plaster and to evaluate the pharmacodynamics.The effects of eth-anol concentration,solvent multiplication and extraction time on the extraction process of Tongshu gel plaster were investigated by using L9(34)3-factor 4-level orthogonal design test method,with the contents of tetrahydropalmatine,paeoniflorin,tetrandrine,and aconitine lac-tone and the extraction dry paste rate as the evaluation criteria.Rat model of cold-congealed blood stasis type dysmenorrhea was established,and rats were divided into four groups:control group,model group,dysmenorrhea ointment group,and Tongshu gel plaster group.The ef-fectiveness of Tongshu gel plaster in treating dysmenorrhea was evaluated by conducting the torsion reaction test in dysmenorrheic rats and using the enzyme-linked immunoassay was to determine the prostaglandin 2α(PGF2α)and prostaglandin(PGE2)in the uterine tissues of rats,and the content of estrogen(E2)and β-endorphin(β-EP)in serum to evaluate the efficacy of Tongshu gel plaster in the treatment of dys-menorrhea.The optimal extraction process of Tongshu gel plaster was as follows:add 8 times the amount of 80％concentration of ethanol in the herbs and soak for 30 min,heat and reflux the extract twice,each time for 2 h.The combined extracts were concentrated under reduced pressure to the appropriate amount and dried under reduced pressure in a vacuum drying oven.The Tongshu gel plaster prepared by the op-timal extraction process parameters could significantly reduce the number of twists and prolong the latency time of twists in dysmenorrheic rats(P＜0.01).Under the effect of Tongshu gel plaster,the content of PGF2α in the uterine tissues of rats decreased and the content of PGE2 increased(P＜0.01),and the content of E2 in serum decreased(P＜0.01)and the content of β-EP increased(P＜0.05).The preferred ex-traction process has high extraction efficiency,good stability and reproducibility and is simple and feasible.This study shows that,Tongshu gel plaster has good ameliorative effect on dysmenorrhea with cold clotting and blood stasis and analgesic efficacy.
关键词
痛舒方凝胶膏剂/痛舒方/提取工艺/痛经/药效学Key words
Tongshu gel plaster/Tongshu recipe/extraction process/dysmenorrhea/pharmacodynamic引用本文复制引用
基金项目
国家自然科学基金(82074025)
黑龙江省中医药局中医药科研课题(ZHY2023-017)
出版年
2024
国家科技期刊平台
NSTL
万方数据