Danshensu Enhances the Protective Effect of Propofol on Ischemia Reper-fusion Injury in Mice Through Wnt-β-catenin Pathway
To explore the mechanism of Danshensu enhancing the protective effect of propofol on renal ischemia-reperfusion injury in mice through Wnt-β-catenin pathway.96 healthy male mice were randomly divided into a blank control group(C group),a sham surgery control group(S group),an ischemia-reperfusion group(IR group),a group treated with propofol after ischemia-reperfusion(IRP group),a group treated with Danshensu after ischemia-reperfusion(IRS group),and a group treated with propofol combined with Danshensu after ischemia-reperfusion(IRPS group),with 16 mice in each group.C group was treated normally,while S group only dissociated bilateral kidneys.The IR group established a mouse ischemia-reperfusion model.The IRP group was treated with propofol 30 minutes before ischemic injury,the IRS group was treated with Danshensu 30 minutes before ischemic injury,and the IRPS group was treated with propofol and Danshensu 30 minutes before ischemic injury.All other treatments were the same as the IR group.Two days after surgery,collect mouse serum and kidney tissue,measure the levels of creatinine(Cr)and urinary nitrogen(BUN)in serum,malondialdehyde(MDA)content and superoxide dismut-ase(SOD)activity in kidney tissue;HE staining was used to observe pathological changes in the kidneys;detect the mRNA and protein lelvel of Wnt4,β-catenin,and Lrp-6 in kidney tissue.The results showed that the levels of Cr,BUN,and MDA in the IR group,IRS group,IRP group,and IRPS group were significantly higher than those in the C and S groups,and the levels of Cr,BUN,and MDA in the IRPS group were significantly lower than those in the IR group,IRP group,and IRS group,with significant differences(P＜0.05);the SOD activity of the IR group,IRS group,IRP group,and IRPS group was significantly lower than that of the C and S groups,and the SOD activity of the IRPS group was significantly higher than that of the IR group,IRP group,and IRS group(P＜0.05);the degree of HE staining renal damage in the IR group,IRS group,IRP group,and IRPS group was significantly higher than that in the C and S groups,and the degree of renal dam-age in the IRPS group was significantly lower than that in the IR group,IRP group,and IRS group(P＜0.05);the mRNA and protein levels of Wnt4,β-catenin,and Lrp-6 in IR group,IRS group,IRP group,IRPS group were significantly higher than those in C and S group,the mRNA and protein levels of Wnt4,β-catenin,and Lrp-6 in IRP group,IRS group,and IRPS group were significantly higher than those in the IR group,and the mRNA and protein levels of Wnt4,β-catenin,and Lrp-6 in IRPS group were significantly higher than those in the IRP group(all P＜0.05).Danshensu can enhance the protective effect of propofol on renal tissue damage caused by ischemia-reperfusion in mice,reducing renal tissue damage,and its mechanism may be through upregulating Wnt-β-catenin pathway achieves renal protection.
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