摘要
本文旨在建立参柏舒心颗粒HPLC指纹图谱.本研究采用Waters C18色谱柱(4.6 mm×250 mm,5 μm);流动相:乙腈-0.05％三氟乙酸水溶液;流速为0.8 mL/min,进行梯度洗脱;柱温为40 ℃;检测波长为288 nm.建立10批参柏舒心颗粒HPLC指纹图谱,利用"中药色谱指纹图谱相似度评价系统(2012版)"软件对其进行相似度评价.建立了参柏舒心颗粒HPLC指纹图谱,共确定了 25个共有峰,鉴定了 5个成分,分别为丹参素钠,原儿茶醛,黄柏碱,丹酚酸B,欧前胡素;10批样品相似度均大于0.93,方法可靠.本研究分析方法具有较强的可行性,达到良好的分离效果,可为提高参柏舒心颗粒生产质量控制提供一定的科学依据.
Abstract
To establish the HPLC fingerprints of Shenbaishuxin granules.The waters C18(4.6 mm×250 mm,5 μm)was used,the mobile phase was acetonitrile-0.05％trifluoroacetic acid solution,the flow rate was 1.0 mL/min with gradient elution,the column temperature was 40 ℃,the detection wavelength was 254 nm.The HPLC fingerprint of ten batches of Shenbaishuxin granules was determined.The simi-larity evaluation was performed by the"Chinese Herbal Chromatographic Similarity Evaluation System(2012 edition)"software.The HPLC fingerprint of Shenbaishuxin granules was established.A total of 25 common peaks were determined and five component were identified.The similarities of ten samples were higher than 0.93,and the method was reliable.This study the analytical method showd feasibility and good separation effect,providing a scientific basis for improving the quality control of Shenbaishuxin granules.
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