The Enrichment of Total Flavonoids from Rhodiola crenulata by Macroporous Resin and Evaluation of Antioxidant Activity
To establish a method for determining the content of total flavonoids in Rhodiola crenulata.Macroporous adsorption resin was used to enrich the total flavonoids in Rhodiola crenulata.The best resin was selected from 5 types of macroporous resins,and the purification process was preliminarily optimized.Determine the antioxidant activity of the enriched total flavonoids in Rhodiola crenulata.Using rutin as a reference substance,the colorimetric method of sodium nitrite-aluminum nitrate reaction was used to determine the total flavonoids con-tent in Rhodiola crenulata.The best macroporous resin was selected using static adsorption-desorption and dynamic adsorption-desorption methods,and the concentration and dosage of eluent were optimized,and the antioxidant activity of total flavonoids in Rhodiola crenulata was determined.The total flavonoids content in Rhodiola crenulata from Tibet is 12.91％,while that in Rhodiola crenulata from Qinghai is 9.85％.D101 macroporous resin was the best resin for enriching total flavonoids in Rhodiola crenulata.The best eluent concentration is 80％ethanol solution,and the best eluent dosage is 5 BV.After purification with D101 macroporous resin,the total flavonoids content in Rhodiola crenulata from Tibet reached 24.64％.Its clearance rate forDPPH free radicals can reach 95.51％.D101 macroporous resin has a good effect on enriching the total flavonoids in Rhodiola crenulata,and the total flavonoids in Rhodiola crenulata have a good antioxidant activity.This study can provide reference for enriching the quality evaluation standards of Rhodiola crenulata and accelerating the development and utili-zation of flavonoids in Rhodiola crenulata.
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