Determination of Five Chemical Components Content in the Classic Formula Danggui Buxue Decoction by HPLC-UV
To establish a HPLC-UV method for the simultaneous determination of five components in Danggui buxue decoction.A diamon-sil C18 column(250 mm × 4.6 mm,5 μm)was used.The mobile phase was methanol(A):0.4％formic acid water gradient elution(B).The detection wavelength was 280 nm.Ferulic acid,SenkyunolideI,Calycosin-7-glucoside,Calycosin-7-glucoside,and Ononin had a good lin-ear relationship in the quality range of 0.091～1.824,0.015～0.308,0.092～1.848,0.036～0.728 and 0.082～1.656 μg/mL,respectively,with the correlation coefficients above 0.999.The limits of quantification were 0.091,0.015,0.092,0.036 and 0.082 μg/mL respectively;The detec-tion limits were about 0.027,0.005,0.028,0.011 and 0.025 μg/mL,respectively.The RSD of precision,repeatability,stability,and the spiked recovery test were less than 5％.The recovery rates were 99.68％,99.40％,100.24％,101.04％,and 100.88％.The method is simple,sensi-tive,rapid,and accurate.It can be used for the content determination and quality control of Danggui buxue decoction.
high performance liquid chromatographyDanggui buxue decoctionferulic acidsenkyunolidelcalycosin-7-o-beta-D-gluco-sidecalycosinononincontent determination
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