Culture and Identification of Embryo Fibroblasts from the Longyan Shan-Ma Duck
To cultivate the duck embryo fibroblasts from Longyan Shan-Ma duck.The 10-day-old duck embryo tissues of Longyan Shan-Ma duck were used for the fibroblast culture by the tissue block method and enzyme digestion method,and the cultured fibroblasts were identified by the immunofluorescence method.The results indicated that the cultured duck embryo fibroblasts showed a typical long spindle shape after 12 h,and the outline of the cells were well-defined and the nucleoli were distinct after 48 h.The 3rd generation fibroblasts were all positive after DAPI and green fluorescence staining with the characteristic of fibroblasts.Fibroblasts were successfully isolated and cul-tured from the embryo tissue of the Longyan Shan-Ma duck,which provides good materials for the late research of molecular biology and cell biology of the Longyan Shan-Ma duck.
国家科技期刊平台
NSTL
万方数据
