Study on Notwork Pharmacological Mechanism of Danshen and Sanqi in the Treatment of Knee Osteoarthritis
To provide theoretical support for the clinical application of Danshen and Sanqi in the treatment of knee osteoarthritis(KO A),an exploration on the potential mechanism was performed using network pharmacology and molecular docking.TCMSP database was used to search the main compounds and related targets.GeneCards,OMIM,and DisGeNET databases were used to search the targets of KOA.The intersection targets of Danshen-Sanqi and KOA were obtained by Venn diagram.The composition-target-disease network and PPI network were constructed by using STRING database and Cytoscape software,and the core components and targets were screened.GO functional analysis and KEGG pathway enrichment analysis were performed through DAVID database.AutoDock software was used for molecular docking.A total of 66 active components and 232 related targets were obtained from Danshen-Sanqi.There were 130 intersection targets.Quercetin,luteolin,and tanshinone ⅡA were the core compounds,PTGS2,ADRB2 and OPRM1 were the core targets.GO functional analy-sis obtained 794 entries and KEGG analysis obtained 141 related pathways.Molecular docking results showed that tanshinone ⅡA had a good affinity with ADRB2.The mechanisms of Danshen-Sanqi treatment for KOA mainly involve cancer pathway,AGE-RAGE signaling pathway,lipid metabolism,fluid shear stress signaling pathways,etc.Tanshinone ⅡA and ADRB2 may be the key component and target.
国家科技期刊平台
NSTL
万方数据
