Effects of the Extract of Ligusticum Wallichii and Gastrodia Elata on the Improvement of Learning and Memory in Vascular Dementia Rats
To investigate the effects and mechanisms of Chuanxiong-Tianma extracts on learning and memory in rats with Vascular cognitive impairment(VCI).A VCI model was established using the two-vessel occlusion method(Two-vessel occlusion,2-VO).After successful modeling,the rats were divided into the model group,positive drug group,high-dose group,low-dose group,and sham operation group.The sham operation and model groups received distilled water,the positive drug group received 0.45 mg/(kg·d)of donepezil hydrochloride,and the high and low-dose groups received extracts at doses of 3 g/(kg·d)and 1.5 g/(kg·d),respectively.After 56 days of treatment,the Morris water maze and social behavior tests were conducted to evaluate the rats'behavior;acetylcholine levels and acetylcholinesterase activity were measured using ELISA.Compared with the sham operation group,the model group exhibited decreased spatial learning memory,ex-ploratory abilities,and social interaction.This was evident as the Morris water maze results showed a significantly prolonged escape latency(P＜0.01),a shortened number of platform crossings(P＜0.01),and the social behavior test results showed significantly reduced contact time and number of contacts(P＜0.05).Acetylcholine expression in the brain was downregulated,and acetylcholinesterase activity was up-regulated(P＜0.05).Compared with the model group,the high-dose group showed significant improvements in spatial learning memory,exploratory abilities,and social interaction,indicated by a significantly shortened escape latency,increased number of platform crossings and time spent on the original platform,and significantly increased social contact time and number of contacts(P＜0.05).The expression level of acetylcholine in the brain was upregulated and that of acetylcholinesterase was downregulated(P＜0.05).Chuanxiong-Tianma ex-tract can intervene in the expression of acetylcholine in rat brains,improving learning and memory abilities in VCI rats.
bilateral common carotid artery ligationvascular cognitive impairmentLigusticum WallichiiGastrodia Elatalearning and memoryacetylcholine
国家科技期刊平台
NSTL
万方数据
