Optimization of Preparation Technology of Five Different Pollen Carriers by Orthogonal Experiment
To optimization the preparation process of sporopollenin microencapsulated carriers for five different pollens.In this study,the protein removal rate was used as an index,including phosphoric acid volume fraction,stirring time and stirring temperature,and then opti-mized by orthogonal test to obtain the best preparation process of sporopollenin,and the maximum extreme deviation method was used to analyze and process the orthogonal test data.The results showed that the best preparation conditions were 100 ℃,18 h and 85％phosphoric acid;the best preparation conditions of tea pollen,sunflower pollen,rose pollen and pine pollen were 70 ℃,18 h and 85％phosphoric acid.However,the main factors affecting the protein removal rate were different:the stirring time and the stirring temperature are the main factors affecting the protein removal rate of the rapeseed pollen;the stirring temperature is the protein removal rate of the sunflower pollen;the stir-ring temperature is the protein removal rate of the rose pollen;the stirring time is the main factor affecting the protein removal rate of the pine pollen.In the validation test,three batches of samples were prepared by the optimal process and ultraviolet-visible spectrophotometry(UV)method measured the protein removal rates.And the results showed that:the protein removal rate in rapeseed pollen was(90.62±0.091)％,(92.01±0.150)％in camellia pollen,(92.56±0.291)％in sunflower pollen,(94.98±0.251)％in rose pollen and(92.56±0.291)％in the pine pollen.The orthogonal experimental analysis provided the optimal scheme for the preparation process of different pollen sporopollenins,and laid a theoretical foundation for the research of sporopollenin microcapsule carriers in drug delivery system.
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