The Study on the Mechanism of IFNα Expression Stimulation by ATG13
Autophagy-related gene 13(atg13)plays a pivotal role in autophagic machinery.However,there is a big unexplored field involved in regulating interferon-α(IFN-α)production.Based on the species-specific lineages for genetic divergence of atg13 genes originated from different organisms,we used atg13 gene originated from Homo sapiens and human embryonic kidney(HEK 293T)cells to investigate the effects of atg13 overexpression on IFN-α signaling pathway.The showed results that atg13 overexpression has stronger induction of IFN-αthan that of IFN-β.Even though atg13 overexpression appears to interact with several important IFN-α signaling molecules(MyD88,IKKα,TRAF6 and IRF7)that the corresponding molecules display small changes at the transcriptional and translational levels to different degrees,ATG13 protein has a strong relationship with IRF7 phosphorylation involved in induction of IFN-α.In addition,there is an interesting phe-nomenon that ATG13 protein is able to trigger activities of interferon-stimulated response element(ISRE).Altogether,ATG13 protein has non-autophagic roles in triggering IRF7 phosphorylation and interacting with ISRE to directly initiate IFN-α production.
Autophagy-related gene 13interferon-αsignaling pathwayphosphorylationinterferon-stimulated response element
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