Salvia miltiorrhiza Bge.Extract Combined with Remifentanil Attenuates Hypoxia/Reoxygenation-induced Cardiomyocyte Injury by Regulating Nrf2 Signaling Pathway
In order to investigate the effect and mechanism of Salvia miltiorrhiza Bge.extract combined with remifentanil in alleviating hy-poxia/reoxygenation-induced myocardial cell injury.H9c2 cardiomyocytes were divided into control group,model group,danshen extract group(Tan Ⅰ group),RPC group and combination group.The cells in the control group were not treated.The myocardial cell injury model was induced by hypoxia/reoxygenation(HR)in the model group.The Tan Ⅰ group was incubated with 1 μmol/L Tan Ⅰ for 24 h on the basis of the model group.The RPC group was incubated with 1μmol/L RPC for 24 h on the basis of the model group.The combined group was incubated with 1 μmol/L Tan Ⅰ and 1 μmol/L RPC for 24 h on the basis of the model group.The cell viability of each group was determined by CCK-8,the apoptosis rate of each group was determined by flow cytometry,and the expression levels of Nrf2 and HO-1 in each group were determined by western blot.Nrf2-siRNA was further transfected to analyze the effect of Nrf2 signaling pathway on the protection of Tan Ⅰ combined with RPC against HR-induced H9c2 cardiomyocyte injury.The viability of H9c2 cardiomyocytes was significantly decre-ased after HR induction.After drug treatment,the cell viability of H9c2 cells in Tan Ⅰ group,RPC group and combination group was signifi-cantly increased,and the improvement of cell viability in combination group was more significant than that in Tan Ⅰ group and RPC group.The apoptosis rate of H9c2 cardiomyocytes increased significantly after HR induction.After drug treatment,the apoptosis rate of H9c2 cells in Tan Ⅰ group,RPC group and combination group decreased significantly,and the decrease of apoptosis rate in combination group was more significant than that in Tan Ⅰ group and RPC group.HR caused a significant decrease in the expression of total Nrf2 protein and nuclear Nrf2 protein in H9c2 cells.After administration,the expression of total Nrf2 protein and nuclear Nrf2 protein was increased,and the Nrf2 protein in the cytoplasm was decreased.Compared with Tan Ⅰ group and RPC group,the expression levels of total Nrf2 protein and nuclear Nrf2 protein in the combined group were significantly increased,and the expression level of Nrf2 protein in the cytoplasm was significantly de-creased.After transfection of Nrf2-siRNA,the expression of Nrf2 and HO-1 in H9c2 cardiomyocytes treated with Tan Ⅰ combined with RPC decreased.Salvia miltiorrhiza extract combined with remifentanil attenuates HR-induced cardiomyocyte injury by modulating the Nrf2 sig-nalling pathway.
Salvia miltiorrhiza Bge.extractremifentanilNrf2 signaling pathwaymyocardial cell injuryhypoxia/reoxygenation
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