Simultaneous Determination of Chlorogenic Acid,Luteolin and Iso-chlorogenic Acid A in Qiju Lutein Ester Granules by HPLC
To establish a method for the simultaneous determination of chlorogenic acid,luteolin glycoside and isochlorogenic acid A in Qiju lutein ester granules.HPLC was performed with Amethyst C18-H(4.6 mm × 250 mm,5 μm)as the chromatographic column,acetonit-rile-0.1％phosphoric acid aqueous solution was selected as the mobile phase for gradient elution,the detection wavelength was 348nm,and the column temperature was 25 ℃,injection volume was 2 μL.Chlorogenic acid,luteolin and isochlorogenic acid A showed good linear relationship with peak area in the range of 0.01～0.07 mg/mL,0.01～0.05 mg/mL and 0.03～0.16 mg/mL,respectively,and the correlation coefficients were 0.999 4,0.999 5 and 0.999 2,respectively;the average recoveries were 101.33％,95.31％and 109.60％,and the relative standard deviations were 1.59％,1.29％and 1.42％,respectively;the relative standard deviations(RSDs)of precision test results were 1.01％,0.73％and 0.45％,respectively;the relative standard deviations(RSDs)of stability test results were 1.49％,1.26％and 0.72％.The average con-tents of chlorogenic acid,luteolin and isochlorogenic acid A in three batches of products were(98.94±0.56)mg/100 g,(90.45±0.82)mg/100 g and(145.63±2.21)mg/100 g(n=9).The method in this study is simple,accurate and reliable,and can be used for the content determination of Qiju lutein ester granules.
万方数据
维普
