Determination of the Contents of Fatty Acids in Hippophae rhamnoides L.by Pre-column Derivatization-GC
To establish a pre-column derivatization-Gas chromatography method for the determination of fatty acids in Hippophae rhamnoides L.The fatty oil in sea buckthorn was extracted by Soxhlet extraction,and the fatty acids in sea buckthorn were determined by Gas chroma-tography after methyl esterification.Results showed that the linear ranges of myristic acid,palmitic acid,palmitoleic acid acid,stearic acid,oleic acid,linoleic acid and α-linolenic acid determined by pre-column derivatization were all better than 0.999 8.The average recoveries of myristic acid,palmitic acid,stearic acid,oleic acid,linoleic acid and α-linolenic acid were all higher than 93.1％,the RSD was less than 2.8％,and the seed,pericarp and pulp of Hippophae rhamnoides were clustered into one group by Spass software,the results showed that the fatty acids of the three medicinal parts could be used as important indexes to distinguish different parts of Hippophae rhamnoides L.The content of linoleic acid in seeds was about twice as high as that in pulp and pericarp,and the content of α-linolenic acid in seeds was more than 15 times as high as that in pulp and pericarp,and the ratio of palmitic acid to palmitoleic acid acid was estimated,the ratio of palmitic acid to palmitoleic acid acid increased with the increase of storage time,and the fatty acids content of Hippophae Fructus fruit was the best after drying after steaming for 20 min.Determination of fatty acids in sea buckthorn by pre-column derivatization and Gas chromatography can be used for quality evaluation of sea buckthorn.
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