Study on the Extraction of Total Flavonoids From Pesudognaphalium affine(D.DON)Anderb.and Their Antioxidant Properties by Ultrasonic-assisted Two-phase Aqueous System
In this experiment,the best technological route of the extraction of total flavonoids by ultrasound-assisted aqueous two-phase from Pesudognaphalium affine(D.DON)Anderb.was explored by using Pesudognaphalium affine(D.DON)Anderb.as raw material and the yield of total flavonoids from Pesudognaphalium affine(D.DON)Anderb.as an index.Using 52×3 two-water phase test method,the optimum composition system of two-water phase was studied based on the yield of total flavonoids of Pesudognaphalium affine(D.DON)Anderb.The results showed that the optimal system composition was 16％dipotassium hydrogen phosphate and 30％ethanol.Compared with the traditional extraction methods of ethanol and hot water extraction,the two-aqueous phase method had better extraction effect,and the extraction rate was increased by 1 to 2 times.Taking the yield of Pesudognaphalium affine(D.DON)Anderb.as the response index,the optimal ultrasonic process parameters were optimized by response surface optimization,including ultrasonic power 420 W,extraction time 23 min,and ultrasonic temperature 50 ℃.Compared with the conventional aqueous two-phase extraction at room temperature,the extraction yield was increased by 34％.The results of the total antioxidant capacity test highlighted that the total antioxidant capacity of the flavonoid extract of the ultrasonic assisted aqueous phase was about 2 times that of the water extract and 1.8 times that of the ethanol extract.In con-clusion,the ultrasound-assisted aqueous two-phase extraction method optimized in this study has a higher extraction efficiency for the fla-vonoids of Pesudognaphalium affine(D.DON)Anderb.,and at the same time,the extract can maintain a stronger antioxidant activity.
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