Determination of Rutin,Quercetin-3-O-sophoroside and Total Flavonoids in Scleromitrion diffusum(wild.)R.J.Wang Injection by HPLC
To establish a HPLC method for the determination of rutin,quercetin-3-O-sophoroside and total flavonoids in Scleromitrion dif-fusum(wild.)R.J.Wang injection.The content of rutin,quercetin-3-O-sophoroside and total flavonoids in Scleromitrion diffusum(wild.)R.J.Wang injection were determined by HPLC on InertSustainSwift C18(4.6mm×250 mm,5 μm)column,acetonitrile(A)-0.1％acetic acid aqueous solution(B)with gradient elution,column temperature 30 ℃,volume flow rate of 1.0 mL/min,detection wavelength 369 nm).The injection amount of rutin had a good linear relationship(R2=0.999 2)and an average recovery rate(n=6)of 97.3％in the range of 0.201 6～3.225 6 μg,a linear relationship of quercetin-3-O-sophoroside in the range of 0.196 0～3.136 0 μg(R2=0.999 3),an average recovery rate of 97.3％,an average recovery rate of total flavonoids(n=6)of 98.9％,and an RSD of 1.46％.The average content of rutin in Sclerom-itrion diffusum(wild.)R.J.Wang injection(n=3)was 0.08 mg/mL.The mean content of quercetin-3-O-sophoroside(n=3)was 0.20 mg/mL and the total flavonoid content was 1.26 mg/mL.The method is accurate,convenient and rapid in this study and provides a new method for the determination of rutin,quercetin-3-O-sophoroside and total flavonoids in Scleromitrion diffusum(wild.)R.J.Wang injection.
万方数据
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