首页|食用纳豆对抗生素性腹泻小鼠肠道稳态的影响

食用纳豆对抗生素性腹泻小鼠肠道稳态的影响

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为探讨纳豆饲料对抗生素性腹泻小鼠肠道菌群结构和肠道稳态失调的保护作用，向C57BL/6小鼠连续饲喂2。5％添加量的纳豆饲料19周后，通过连续灌胃复合抗生素7 d[110 mg/mL，0。4 mL/(d·只)]建立抗生素性腹泻模型(Antibiotic-associated diarrhea，AAD)，随后停用抗生素，恢复自由饮食7 d。测定造模后和恢复后小鼠生理指标，肠道损伤情况和肠道菌群结构功能的变化。结果显示，食用纳豆能够缓解AAD小鼠的体重降低，结肠缩短和结肠病理损伤，还可以通过改善血清炎症因子、血清内毒素和结肠免疫球蛋白水平的异常，升高结肠SOD含量，降低结肠MDA含量，减轻AAD小鼠炎症反应和氧化损伤。此外，食用纳豆增加了 AAD小鼠结肠短链脂肪酸水平，增加了 Bacillus、Enterococcus、Allobaculum等有益菌丰度。这些菌群结构的变化与纳豆改善的AAD小鼠病理指标显著相关，且显著加速了 AAD小鼠的指标恢复。本研究结果表明，食用纳豆能够对AAD小鼠的氧化应激、炎症水平、肠道菌群结构和肠道稳态产生有益作用，从而保护小鼠抵御抗生素造成的肠道损伤。
Effect of Natto Consumption on Intestinal Homeostasis in Mice with Antibiotic Diarrhea
To investigate the protective effect of natto feed on intestinal flora and intestinal homeostasis in mice with antibiotic-induced di-arrhea.C57BL/6 mice were fed with 2.5％natto for 19 weeks,and the model of antibiotic-induced diarrhea(AAD)was established by in-tragastric administration of compound antibiotics for 7 days[110 mg/mL,0.4 mL/(d·per)],antibiotics were then stopped and the free diet resumed for 7 days.The physiological indexes,intestinal injury and the changes of intestinal flora structure and function were determined after modeling and recovery.The results showed that long-term consumption of natto could alleviate the weight loss,colon shortening and pathological damage in AAD mice.The natto diet also increases colon SOD levels by improving abnormal levels of serum inflammatory factors,endotoxin and colonic immunoglobulins,decreasing the content of MDA in AAD mice colon.More importantly,long-term con-sumption of natto increased colon short-chain fatty acid levels and increased the abundance of beneficial bacteria such as Bacillus,Enteroc-occus and Allobaculum in AAD mice.The changes of these flora structures are significantly related to the pathological indexes of AAD mice improved by natto,and natto significantly accelerates the recovery of AAD mice.Long-term consumption of natto can have beneficial effects on oxidative stress,inflammation level,intestinal flora structure and intestinal homeostasis in AAD mice,thus protecting mice from intest-inal injury caused by antibiotics.

nattooxidative stressinflammatory factorsantibiotic diarrheaintestinal homeostasis

黄伟、张雪聪、何宇光、苗欣宇、孙慕白、牛红红、李达、苏颖、甘雪彤、华梅、王景会

展开 >

吉林师范大学生命科学学院,吉林 四平 136000

吉林省农业科学院(中国农业科技东北创新中心)农产品加工研究所,吉林长春 130033

吉林农业大学食品科学与工程学院,吉林 长春 130033

纳豆 氧化应激 炎症因子 抗生素性腹泻 肠道稳态

吉林省农业科学院基本科研经费项目吉林省农业科技创新工程项目引进博士及高层次项目吉林省农业科技创新工程项目研究生基金吉林省农业科技创新工程项目研究生基金

KYJF2023JJ103CXGC2022RCG001CXGC2022RCY023CXGC2022RCY026

2024

10.16720/j.cnki.tcyj.2024.155

特产研究
中国农业科学院特产研究所,中国农学会特产学会

特产研究

CSTPCD
影响因子：0.331
ISSN：1001-4721
年,卷(期)：2024.46(5)