首页|避瘟解毒颗粒对LPS诱导急性肺损伤小鼠保护作用的研究

避瘟解毒颗粒对LPS诱导急性肺损伤小鼠保护作用的研究

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  • 国家科技期刊平台
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探究避瘟解毒颗粒对脂多糖(LPS)致急性肺损伤(ALI)小鼠的保护作用。将30只C57小鼠随机分为正常组、模型组、地塞米松组、避瘟解毒颗粒高、低剂量组，每组6只，每天给药1次，连续给药7 d。模型组、地塞米松组、避瘟解毒颗粒给药组气管滴注脂多糖(LPS)构建小鼠急性肺损伤模型(ALI)。造模后72 h取材检测，分析小鼠体重变化情况;血常规指标;胸腺指数;ELISA法检测肺泡灌洗液(BALF)中IL-1β、IL-6、TNF-α水平;HE染色法观察肺组织病理变化。与模型组比较，避瘟解毒颗粒高剂量组小鼠血清中血常规指标BALF中炎性细胞因子IL-6、TNF-α含量，肺组织损伤程度均有不同程度的降低(P＜0。05，P＜0。01)，且高剂量组能显著升高ALI小鼠的胸腺指数(P＜0。01)。避瘟解毒颗粒对LPS诱导急性肺损伤发挥一定的保护作用，改善小鼠肺组织的病理改变，增强免疫功能，其作用机制可能与抑制促炎细胞因子的分泌及发挥抗炎作用有关。
Protective Effect of BiWen Jiedu Granules on LPS-induced Acute Lung Injury in Mice
To explore the effect BiWen Jiedu Granules on LPS-induced acute lung injury in mice.Thirty male C57 mice were randomly di-vided into the normal group,the model group,the dexamethasone group,and the low-dose and high-dose groups of BiWen Jiedu Granules,with 6 in each group,which were administered once a day for 7 days.The acute lung injury model was established by tracheal infusion of lipopolysaccharide(LPS)in the model group,the dexamethasone group,and the low-dose and high-dose groups of BiWen Jiedu Granules group.The material was taken at 72 h,the rats had their body weights,blood routine detected,their bronchoalveolar lavage fluid(BALF)lev-els of IL-1β,IL-6 and TNF-α detected by ELISA;the lung histopathological changes were observed by the HE staining.Compared with the modeld group,the the high-dose BiWen Jiedu Granules group shared lower degree of blood routine,levels of inflammatory cytokine IL-6 and TNF-αin BALF,lung injury(P＜0.05,P＜0.01),high-dose groups can increase the thymus index of ALI mice obviously(P＜0.01).BiWen Jiedu Granules plays a protective role in LPS-induced acute lung injury in mice,improve the histopathological changes of lung,enhance immune function and its mechanism may be related to inhibiting the secretion of inflammatory cytokines function.

BiWen Jiedu Granuleslipopolysaccharideacute lung injuryinflammatory cytokine

王园、杨允、王博、崔妮、王海峰、张红、刘洋、龙凯花、卫向锋

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陕西省中医药研究院,陕西 西安 710003

西北大学,陕西 西安 710069

西安中医脑病医院,陕西 西安 710032

西安市中药制剂工程技术研究中心,陕西 西安 710032

武警陕西省总队医院,陕西 西安 710000

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避瘟解毒颗粒 脂多糖 急性肺损伤 炎症因子

陕西省重点研发计划项目秦创原中医药创新研发转化项目"秦药"研发重点实验室"秦药"质量提升工程项目陕西省科协青年人才托举计划项目西安市科技计划项目陕西省科技厅一般项目

2022ZDXM-SF-062022-QCYZH-0062021-QYPT-0012021-QYZL-012023032123YXYJ01422024SF-YBXM-489

2024

10.16720/j.cnki.tcyj.2024.158

特产研究
中国农业科学院特产研究所,中国农学会特产学会

特产研究

CSTPCD
影响因子：0.331
ISSN：1001-4721
年,卷(期)：2024.46(5)