摘要
本研究建立同时分析12种人参皂苷的超高效液相-三重四极杆串联质谱(UPLC-MS)分析方法,比较人参果自然放置发酵前后的12种人参皂苷含量变化.将样品经醇回流提取,采用Thermo Syncronis-C18色谱柱(100 mm×2.1 mm,1.7μm),0.1％甲酸-水溶液(A)-乙腈(B)为流动相,采用梯度洗脱,流速0.2 mL/min;柱温30 ℃;进样量5 μL;电喷雾离子源(ESI);喷雾电压2.5 kV;负离子扫描模式;扫描范围m/z 100.0～1 500.0;采用Xcalibur分析软件处理数据.结果表明,人参皂苷的线性关系良好(R2≥0.995),平均回收率在91.23％～109.13％,稳定性RSD值为0.95％～2.67％,重复性RSD值为1.37％～2.67％.人参果中人参皂苷含量随着放置发酵时间的不同,含量会发生变化,此分析方法可用于人参果中的人参皂苷含量测定,对于人参果质量控制具有指导作用.
Abstract
This study established an ultra performance liquid chromatography triple quadrupole tandem mass spectrometry(UPLC-MS)method for simultaneous analysis of 12 ginsenosides,and to compare the content changes of 12 ginsenosides in ginseng berry before and after natural fermentation.The sample was extracted by alcohol reflux,and a Thermo Synchronis-C 18 chromatographic column(100 mm × 2.1 mm,1.7 μm)using 0.1％formic acid aqueous solution(A)and acetonitrile(B)as the mobile phase and gradient elution at a flow rate of 0.2 mL/min;column temperature:30 ℃;injection volume 5 μL;electric spray ion source(ESI);spray voltage 2.5 kV;negative ion scanning mode;scan-ning range:m/z 100.0～1500.0;the data were processed using Xcalibur analysis software.The linear relationship among ginsenosides was good(R2≥ 0.995),with an average recovery of 91.23％to 109.13％,a stability RSD of 0.95％to 2.67％,and a repeatability RSD of 1.37％to 2.67％.The contents of ginsenosides in Ginseng berry changed with the time of storage and fermentation.This analytical method could be used to determine the content of ginsenosides in ginseng berry,and has a guiding role in the quality control of ginseng berry.
基金项目
大学生创新创业训练项目(202110199015)
国家科技期刊平台
NSTL
万方数据