Analysis of 12 Ginsenosides in Ginseng Berry Fermented at Different Time by UPLC-MS
This study established an ultra performance liquid chromatography triple quadrupole tandem mass spectrometry(UPLC-MS)method for simultaneous analysis of 12 ginsenosides,and to compare the content changes of 12 ginsenosides in ginseng berry before and after natural fermentation.The sample was extracted by alcohol reflux,and a Thermo Synchronis-C 18 chromatographic column(100 mm × 2.1 mm,1.7 μm)using 0.1％formic acid aqueous solution(A)and acetonitrile(B)as the mobile phase and gradient elution at a flow rate of 0.2 mL/min;column temperature:30 ℃;injection volume 5 μL;electric spray ion source(ESI);spray voltage 2.5 kV;negative ion scanning mode;scan-ning range:m/z 100.0～1500.0;the data were processed using Xcalibur analysis software.The linear relationship among ginsenosides was good(R2≥ 0.995),with an average recovery of 91.23％to 109.13％,a stability RSD of 0.95％to 2.67％,and a repeatability RSD of 1.37％to 2.67％.The contents of ginsenosides in Ginseng berry changed with the time of storage and fermentation.This analytical method could be used to determine the content of ginsenosides in ginseng berry,and has a guiding role in the quality control of ginseng berry.
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