Determination of Vanillic Acid and Ferulic Acid in Iron Chopstick by HPLC-DAD Method
A high performance liquid chromatography(HPLC)-Diode Array Detector(DAD)method was established for the determina-tion of vanillic acid and ferulic acid in iron chopsticks herbs.The chromatography was performed on ZORBAX Eclipse Plus C18 column(4.6 mm× 250 mm,5 μm).The mobile phase was acetonitrile(A)-0.2％phosphoric acid water(B),gradient elution,flow rate 1.0 mL/min;column temperature 25℃;detection wavelength(0～15 min,280 nm,vanillic acid;15-70 min,327 nm,ferulic acid);the injection volume was 10 μL.The separation degree of vanillic acid and ferulic acid was more than 1.5,and the separation effect was good,and the linear rela-tionship was good in the range of 22.72～727.00 μg/mL and 9.69～310.00 μg/mL,respectively,with r＞0.999.The average recoveries(RSD)were 101.08％(3.09％)and 98.98％(3.11％),respectively.The contents of ferulic acid and vanillic acid in iron chopsticks herbs S1 were the highest,the vanillic acid content in S1 was(0.77±1.42)mg/g and ferulic acid content was(0.36±1.32)mg/g.This study established HPLC-DAD switching wavelength method is sensitive,accurate and reproducible,and can be used for the determination of vanillic acid and ferulic acid in the iron chopsticks herbs.
iron chopsticks herbsvanillic acidferulic acidHPLC-DAD switching wavelength method
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