Mechanism of Sanqi Saponins Regulating miR-22 on Lipid Metabolism and Oxidative Stress in Hyperlipidemic Rats with Coronary Heart Disease
The aim of this study was to investigate whether total saponins of Panax notoginseng can regulate miR-22 to regulate lipid metab-olism and oxidative stress in rats with hyperlipidemia and coronary heart disease.60 rats were randomly divided into 6 groups:Blank group,HLP+CHD group,PNS group,miR-NC group,miR-22 group and PNS+anti-miR-22 group(10 rats/group).The heart function of rats was de-tected.The relative expression of miR-22 in myocardial tissue was detected by RT-PCR.Blood lipid index and oxidative stress index were de-tected.Myocardial histopathological changes were detected by HE staining.The expression of Nrf2 and HO-1 proteins in cardiac tissue was de-tected by western blot.The results show that the relative expression of miR-22,LVEF,serum HDL-C level,SOD,GSH-Px,CAT level and Nrf2 and HO-1 protein expression in cardiac tissue of HLP+CHD group and miR-NC group were lower than those of Blank group.LVESD,LVEDD.serum TC,TG,LDL-C and MDA levels were higher than those in Blank group(P＜0.05).Compared with HLP+CHD group,the relative ex-pression level of miR-22,LVEF,serum HDL-C level,SOD,GSH-Px,CAT level andNrf2 and HO-1 protein expression in myocardial tissue of PNS group and miR-22 group were significantly increased.The levels of LVESD,LVEDD,serum TC,TG,LDL-C and MDA were significantly decreased(P＜0.05).The relative expression level of miR-22,LVEF,serum HDL-C level,SOD,GSH-Px,CAT level andNrf2 and HO-1 protein expression in myocardial tissue of PNS+anti-miR-22 group were lower than those of PNS group.The levels of LVESD,LVEDD,serum TC,TG,LDL-C and MDA were higher than those of PNS group(P＜0.05).Panax notoginseng saponins can improve lipid metabolism disorder and oxidative stress in hyperlipidemia rats with coronary heart disease by regulating the level of miR-22.
万方数据
维普
