Novel Extraction Technique for D-Limonene from Dried Tangerine Peel and Its Activity Research
This study aimed to explore the feasibility of using effervescent salts assisted with β-cyclodextrin for the extraction of D-limonene from dried tangerine peel,and to investigate the anti-inflammatory and antioxidant activities of D-limonene.The experiment utilized effer-vescent salts,β-cyclodextrin,and potassium sulfate as auxiliary extraction reagents,employing aqueous two-phase extraction to extract D-limonene from dried tangerine peel,and optimized the extraction conditions.The antioxidant capacity of D-limonene was evaluated using ABTS radical scavenging assay and ferric reducing antioxidant power assay,while its anti-inflammatory ability was assessed using LPS-stimulated RAW 264.7 cells.The results showed that the mass fraction of D-limonene extracted,quantified by gas chromatography,was 3.29％,which is superior to traditional extraction methods.D-limonene demonstrated in vitro antioxidant capacity comparable to positive controls and exhibited significant inhibitory effects on NO,TNF-α,IL-6,and IL-1 β.Effervescent salts assisted with β-cyclodextrin signifi-cantly improved the extraction rate of D-limonene,and D-limonene exhibited notable anti-inflammatory and antioxidant activities,providing a theoretical basis for the subsequent development and utilization of dried tangerine peel.
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