In this study,Phellodendron amurense and Polygonum cuspidatum were identified by TLC.To establish content determination of berberine hydrochloride in Qianshu'an capsules;it provides the basis for the quality control of Qianshu'an capsule.Determination of berber-ine hydrochloride content in Phellodendron amurense by HPLC on YMC-Pack Pro C18(4.6 mm× 150 mm,5 μm),acetonitrile-0.05 mol/L potassium dihydrogen phosphate(25:75)as mobile phase;The column temperature is 40 ℃;The detection wavelength is 347 nm.The results showed that the linear range of berberine hydrochloride determined by HPLC ranges from 9.2 to 147.6 μg/mL,the linear relationship is good,R=0.999 5,the average recovery was 100.42％,RSD was 0.66％the TLC identification spots of Phellodendron amurense and Polygonum cuspidatum were clear and specific.The method for identification and content determination can be used for quality control of Qianshu'an capsule.
维普
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