摘要
本研究开发了薄层色谱法对关黄柏及虎杖药材的鉴别方法,并建立了盐酸小檗碱含量的测定方法,为前舒安胶囊的质量控制提供依据.采用薄层色谱鉴别关黄柏及虎杖药材;高效液相色谱法测定关黄柏中盐酸小檗碱的含量,色谱柱为YMC-Pack Pro C18(4.6 mm× 150 mm,5 μm),以乙腈:0.05 mol/L磷酸二氢钾(25:75)为流动相,柱温为40 ℃,检测波长为347 nm.结果表明,通过高效液相测定的盐酸小檗碱在9.2～147.6μg/mL的范围内,线性关系良好R=0.999 5;平均回收率为100.42％,RSD为0.66％,关黄柏、虎杖的薄层色谱鉴别斑点清晰,专属性强.本研究建立的方法简便、准确、重现性和稳定性均良好,检测成本低,检测结果分析快速,可更有效地控制前舒安胶囊的质量.
Abstract
In this study,Phellodendron amurense and Polygonum cuspidatum were identified by TLC.To establish content determination of berberine hydrochloride in Qianshu'an capsules;it provides the basis for the quality control of Qianshu'an capsule.Determination of berber-ine hydrochloride content in Phellodendron amurense by HPLC on YMC-Pack Pro C18(4.6 mm× 150 mm,5 μm),acetonitrile-0.05 mol/L potassium dihydrogen phosphate(25:75)as mobile phase;The column temperature is 40 ℃;The detection wavelength is 347 nm.The results showed that the linear range of berberine hydrochloride determined by HPLC ranges from 9.2 to 147.6 μg/mL,the linear relationship is good,R=0.999 5,the average recovery was 100.42％,RSD was 0.66％the TLC identification spots of Phellodendron amurense and Polygonum cuspidatum were clear and specific.The method for identification and content determination can be used for quality control of Qianshu'an capsule.
维普
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