首页|Credible and Facile Fluorometric Detection of Soybean Trypsin Inhibitor Activity with a Water-Soluble Poly(diphenylacetylene)Derivative
Credible and Facile Fluorometric Detection of Soybean Trypsin Inhibitor Activity with a Water-Soluble Poly(diphenylacetylene)Derivative
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Herein,a novel method for fluorometric detection of soybean trypsin inhibitor(SBTI)activity based on a water-soluble poly(diphenylacetylene)derivative was reported.Fluorescence quenching of the polymer via p-nitroaniline,produced from the trypsin-catalyzed decomposition of N-benzoyl-DL-arginine-4-nitroanilide hydrochloride(L-BAPA),was well described using the Stern-Volmer equation.SBTI activity was quantitatively assessed based on changes in the fluorescence intensity of the polymer.This strategy has several advantages,such as high sensitivity and ease of operation.Moreover,its applicability to other biochemical analyses is promising.
Zhejiang Xianghe Track Fastener Research Institute,Taizhou 317200,China
Shaoxing Institute for Food and Drug Control,Shaoxing 312000,China
Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province,School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,China
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