MCM4 promotes the proliferation of clear cell renal cell carcinoma cells
Objective:To investigate the effects of minichromosome maintenance protein 4(MCM4)on clear cell renal cell carcinoma(ccRCC)cells.Methods:GEPIA database was used to analyze the expression of MCM4 gene in different tumors,and immunohisto-chemical staining(IHC)was used to detect the expression of MCM4 in ccRCC pathological specimens.HTB-47 and CRL-1932 cells were divided into experimental and control groups,and the effect of MCM4 on cell growth were verified using RT-PCR,WB,cell plate clone formation assay,and CCK-8 assay.We established a co expression network of PCNA and MCM4 using the GEPIA database,and validated the correlation between MCM4 and proliferating cell nuclear antigen(PCNA)in ccRCC using IHC.Results:The GEPIA database showed the MCM4 protein was expressed in ccRCC tissues,and its high expression suggested poor prognosis(P<0.001).The IHC showed that MCM4 expression in ccRCC was higher than that in normal tissues,and clinical features of patients showed a correlation between tu-mor size and MCM4 expression levels(χ2=9.199,P<0.05).Knocking down the MCM4 gene resulted in a decrease in MCM4 protein ex-pression(T=5.432,4.784,all P<0.05)and cell proliferation ability in HTB-47 and CRL-1932 cells(T=4.798,5.278,5.112,4.628,all P<0.05).The high and low expression of MCM4 was consistent with PCNA expression in both the database and IHC(R=0.64,χ2=8.414,all P<0.05).Conclusion:MCM4 is highly expressed in ccRCC and is associated with adverse clinicopathological features.Knocking down the MCM4 gene can inhibit the proliferation of ccRCC cells,and its expression is highly consistent with PCNA gene expression.
万方数据
维普
