Proteomic analysis of aqueous humor in Uygur and Han cataracts patients with pseudoexfoliation syndrome
Objective:To analyze the difference of aqueous humor protein expression in pseudoexfoliation syndrome cataract(PXEC)between Uygur and Han patients.Methods:Six aqueous humor samples were collected from Xinjiang Uygur PXEC patients undergoing surgical treatment in Hotan Regional People's Hospital(group U)and Han PXEC patients(group H)undergoing surgical treatment in the Eye Hospital of Tianjin Medical University from June 2020 to January 2021,respectively.Non-label quantitative proteomics was used to identify and quantitatively analyze the proteins in aqueous humor of the two groups,the differential proteins were screened out,and the functions and regulatory signaling pathways of the differential proteins in aqueous humor of the two groups were analyzed by gene ontolo-gy(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway.The protein-protein interaction(PPI)network was established using STRING database,and MCODE plug-in in Cytoscape software was used to identify the hub pro-teins in PPI network.Results:In this study,a total of 629 proteins were identified.A total of 123 differentially expressed proteins were screened with the difference ratio＞2 and P<0.05.Compared with group H,92 proteins were down-regulated and 31 proteins were up-regulated in group U.Gene Ontology(GO)analysis and the Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis of these di-fferential proteins focused on the complement and coagulation grade pathway,extracellular matrix receptor interaction pathway,systemic lupus erythematosus pathway,focal adhesion pathway,amoebiasis pathway,platelet activation pathway,lysosomal pathway,cholesterol metabolism pathway,and various types of N-Chitosan biosynthesis and other pathways.17 hub proteins were screened through PPI net-work,including fibronectin(FN1),apolipoprotein E(ApoE),amyloid beta protein precursor(APP),vitronectin(VTN),cluster(CLU),apolipoprotein A-Ⅳ(ApoA4),complement C4A(C4A),complement C4B(C4B),Coagulation factor XII(F12),fibrinogen alpha chain(FGA),fibrinogen beta chain(FGB),fibrinogen gamma chain(FGG),kininogen 1(KING1),histidine-rich glycoprotein(HRG),com-plement C8A(C8A),retinol-binding protein 4(RBP4),andHeparin cofactor 2(SERPIND1),with an MCODE Score of 12.375.Conclusion:The 17 hub proteins,including ApoA4,FGA,FGB and FGG,are mainly involved in complement and clotting pathway and cholesterol metabolism pathway,suggesting that there are biological differences in cholesterol metabolism and inflammation regula-tion between the two ethnic groups.
万方数据
维普
