Purification and biological activity of Tremella fuciformis polysaccharides
In order to obtain a purer Tremellafuciformis polysaccharide(TFPS),the crude Tremellafuciformis polysaccha-ride(CTFPS)is purified with AB-8 macroporous resin method,enzymatic method,trichloroacetic acid(TCA)method,and glucono-delta-lactone(GDL)method,respectively.Based on the loss rate of polysaccharide and re-moval rate of protein,the method with the best deproteinization effect was selected from five different depro-teinization methods,and the process conditions for deproteinization of TFPS were optimized through single factor experiments.The structure of TFPS before and after deproteinization was analyzed using IR and SEM.Mean-while,the bioactivity of TFPS before and after deproteinization was compared through the antioxidant activity and moisture absorption and moisturizing property tests.The results showed that adsorption method with AB-8 macro-porous resin was the best for protein removal,and the optimal process conditions were aqueous concentration of CTFPS of 5 mg/mL,dosage of AB-8 macroporous resin of 100 mg/mL,adsorption time of 5 h,and adsorption temperature of 20 ℃.Under these conditions,the removal rate of protein was 82.47％and the loss rate of polysaccharide was 18.24％.Moreover,the antioxidant activity,moisture-absorbing and moisturizing properties of TFPS after deproteinization were stronger than those without deproteinization.
万方数据
维普
