菌株ZYRH-19的筛选鉴定及其合成韦兰胶的特性研究
Screening,identification of strain ZYRH-19 and characteristics of its product welan gum
章阳 1陆贺港 1游鹏智 1李晓雁 2黄海东1
作者信息
- 1. 天津农学院农学与资源环境学院,天津 300392
- 2. 天津农学院食品科学与生物工程学院,天津 300392
- 折叠
摘要
从土壤中分离得到一株产胞外多糖的菌株ZYRH-19,从形态学、生理生化和16SrDNA基因序列的角度对其进行分类鉴定,并对其发酵合成的胞外多糖进行分析鉴别.结果表明:ZYRH-19为鞘氨醇单胞菌属菌株,与Sphingomonas sp.ATCC 31555同源性为99.93％,其合成的胞外多糖为韦兰胶,重均分子量为5.3×106u;常温下韦兰胶溶液具有良好的稀释剪切性,在6r/min剪切率下黏度能达到1 444.1 mPa·s,凝胶强度为22.6 g,在160 ℃下具有良好的抗温性能.研究表明,菌株ZYRH-19合成的韦兰胶性能更好,为韦兰胶的工业生产提供了新的菌种资源.
Abstract
In this study,a strain ZYRH-19 producing extracellular polysaccharide was isolated from soil.It was classified and identified from the perspectives of morphology,physiology,biochemistry,and 16S rDNA gene sequence,and the extracellular polysaccharide synthesized by strain ZYRH-19 was analyzed and identified.The results showed that ZYRH-19 was a strain of Sphingomonas sp.,and its homology with Sphingomonas sp.ATCC 31555 was 99.93％.The extracellular polysaccharide synthesized by strain ZYRH-19 was welan gum with weight average molecular weight of 5.3 × 106u.The welan gum solution synthesized by ZYRH-19 had good dilution shear property at room temperature.The viscosity of the product solution could reach 1 444.1 mPa·s at the 6 r/min shear rate,and its gel strength was 22.6 g.It had good temperature resistance at 160 ℃.The results showed that the performance of welan gum synthesized by strain ZYRH-19 is very good,which provides a new strain resource for the industrial production of welan gum.
关键词
鞘氨醇单胞菌/分类鉴定/韦兰胶/多糖Key words
Sphingomonas/taxonomic identification/welan gum/polysaccharide引用本文复制引用
基金项目
国家级大学生创新创业训练计划(202110061040)
国家自然科学基金面上项目(31571790)
出版年
2024
NETL
NSTL
万方数据