首页|Ag+及激素对藜麦愈伤诱导及分化的影响

Ag+及激素对藜麦愈伤诱导及分化的影响

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本研究以'津藜2 号'藜麦(Chenopodium quinoa)种子为材料,采用MS培养基研究不同浓度Ag+、NAA和蔗糖对藜麦愈伤组织诱导和分化的影响.结果表明,在培养基MS+30 g/L蔗糖+20 μmol/L Ag++0.8 mg/L NAA中藜麦愈伤组织诱导率最高,可达到 100.0%.在培养基MS+40 g/L蔗糖+20 μmol/L Ag++0.4 mg/L NAA中其愈伤组织诱导率最低,只有 13.3%.在培养基MS+30 g/L蔗糖+20 μmol/L Ag++1.5 mg/L 6-BA中其愈伤组织分化率最高,可达到 100.0%.在培养基MS+20 g/L蔗糖+20 μmol/L Ag++2.0 mg/L 6-BA中其愈伤组织分化率最低,只有 20.0%.藜麦最适愈伤组织诱导和分化培养基配方的筛选为其再生体系和转化体系的建立奠定基础,也为其种质资源保存、培育新品种以及工厂化育种提供科学依据.
Effects of Ag ions and hormones on callus induction and differentiation of Chenopodium quinoa
In this study,the effects of different concentrations of NAA hormone and different concentrations of sucrose on the callus of Chenopodium quinoa No.2 under different concentrations of Ag ions were studied based on MS medium.The results showed that in the medium MS+30 g/L sucrose+20 μmol/L Ag++0.8 mg/L NAA,C.quinoa No.2 seeds had the highest callus induction rate,reaching 100%.In medium MS+40 g/L sucrose+20μmol/L Ag++0.4 mg/L NAA,C.quinoa No.2 seed had the lowest callus induction rate,only 13.3%.In medium MS+30 g/L sucrose+20 μmol/L Ag++1.5 mg/L 6-BA,the differentiation rate of C.quinoa No.2 callus was the highest,which could reach 100%.In medium MS+20 g/L sucrose+20 μmol/L Ag++2.0 mg/L 6-BA,C.quinoa No.2 callus had the lowest differentiation rate,only 20.0%.In this study,the optimal induction medium and optimal differentiation medium formula of C.quinoa No.2 callus were obtained,which will lay the foundation for the establishment of quinoa regeneration system and transformation system,and provide scientific basis for the preservation of quinoa germplasm resources,the cultivation of new varieties and factory breeding.

Chenopodium quinoatissue culturecallusinductiondifferentiation

李红、潘治锦、谢晓东、金瑞、曹高燚、陈小强

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天津农学院 农学与资源环境学院,天津 300392

藜麦 组织培养 愈伤组织 诱导 分化

天津市大学生创新创业训练计划项目天津市科技计划项目科技援助项目天津市科技计划项目种业创新重大专项天津市中央引导地方科技发展资金项目天津市奶牛(肉羊)现代农业产业技术体系(奶牛粗饲料生产)天津市科技局优秀企业科技特派员项目天津市自然科学基金

20221006114422ZYCGSN0002022ZXZYSN0002021ZYCGSN00130ITTCRS20210000621YDTPJC0041022JCQNJC01470

2024

10.19640/j.cnki.jtau.2024.03.006

天津农学院学报
天津农学院

天津农学院学报

影响因子：0.612
ISSN：1008-5394
年,卷(期)：2024.31(3)