摘要
本研究以'津藜2 号'藜麦(Chenopodium quinoa)种子为材料,采用MS培养基研究不同浓度Ag+、NAA和蔗糖对藜麦愈伤组织诱导和分化的影响.结果表明,在培养基MS+30 g/L蔗糖+20 μmol/L Ag++0.8 mg/L NAA中藜麦愈伤组织诱导率最高,可达到 100.0%.在培养基MS+40 g/L蔗糖+20 μmol/L Ag++0.4 mg/L NAA中其愈伤组织诱导率最低,只有 13.3%.在培养基MS+30 g/L蔗糖+20 μmol/L Ag++1.5 mg/L 6-BA中其愈伤组织分化率最高,可达到 100.0%.在培养基MS+20 g/L蔗糖+20 μmol/L Ag++2.0 mg/L 6-BA中其愈伤组织分化率最低,只有 20.0%.藜麦最适愈伤组织诱导和分化培养基配方的筛选为其再生体系和转化体系的建立奠定基础,也为其种质资源保存、培育新品种以及工厂化育种提供科学依据.
Abstract
In this study,the effects of different concentrations of NAA hormone and different concentrations of sucrose on the callus of Chenopodium quinoa No.2 under different concentrations of Ag ions were studied based on MS medium.The results showed that in the medium MS+30 g/L sucrose+20 μmol/L Ag++0.8 mg/L NAA,C.quinoa No.2 seeds had the highest callus induction rate,reaching 100%.In medium MS+40 g/L sucrose+20μmol/L Ag++0.4 mg/L NAA,C.quinoa No.2 seed had the lowest callus induction rate,only 13.3%.In medium MS+30 g/L sucrose+20 μmol/L Ag++1.5 mg/L 6-BA,the differentiation rate of C.quinoa No.2 callus was the highest,which could reach 100%.In medium MS+20 g/L sucrose+20 μmol/L Ag++2.0 mg/L 6-BA,C.quinoa No.2 callus had the lowest differentiation rate,only 20.0%.In this study,the optimal induction medium and optimal differentiation medium formula of C.quinoa No.2 callus were obtained,which will lay the foundation for the establishment of quinoa regeneration system and transformation system,and provide scientific basis for the preservation of quinoa germplasm resources,the cultivation of new varieties and factory breeding.
基金项目
天津市大学生创新创业训练计划项目(202210061144)
天津市科技计划项目科技援助项目(22ZYCGSN00020)
天津市科技计划项目种业创新重大专项(22ZXZYSN00020)
天津市中央引导地方科技发展资金项目(21ZYCGSN00130)
天津市奶牛(肉羊)现代农业产业技术体系(奶牛粗饲料生产)(ITTCRS202100006)
天津市科技局优秀企业科技特派员项目(21YDTPJC00410)
天津市自然科学基金(22JCQNJC01470)
NETL
NSTL
万方数据