Soluble dietary fiber extraction and structural characterization of old stems of asparagus and functional studies
In order to study the structural characterization and functionality of soluble dietary fiber(SDF)of asparagus old stem,the old stem of asparagus was treated by cellulase enzymatic digestion,and SDF was extracted by enzymatic method(amylase,glycosylase),and the extraction process was optimized by univariate and response surface analysis,and the microstructure and function of SDF were studied.The results showed that the optimal extraction process of SDF of asparagus old stem after cellulase enzymatic digestion was 0.5%of amylase addition,0.20%of glycosylase addition,2 h enzymatic digestion time,and 65℃.Enzymatic SDF(Ms)in comparison with unenzed SDF(Ws)under this extraction condition.After infrared spectral scanning,both Ms and Ws had typical polysaccharide characteristics.Electron microscopy scanning showed that the Ms structure had a larger surface area and was honeycomb-shaped than Ws.Compared with Ws,Ms had 1.2 times more water retention,1.55 times more oil holding,and stronger thermal stability.The results of this study can provide corresponding theoretical basis for the reuse of soluble dietary fiber in asparagus old stems.
old stems of asparagussoluble dietary fiberextractionstructural characterizationfunctionality
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