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银耳酵素体外消化对抗氧化活性的影响

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为研究体外消化对银耳酵素抗氧化活性的影响,以酵母菌、乳酸菌、酵母菌和乳酸菌混合菌种发酵的 3 种银耳酵素为消化对象,以·OH清除率、DPPH清除率、·O2-清除率和铁原子还原力为抗氧化性指标,分析它们在体外模拟消化过程中抗氧化能力的变化规律.3 种银耳酵素在体外胃肠消化过程中,除了酵母发酵银耳酵素的DPPH清除能力在肠消化阶段有降低外,其余的抗氧化性均增强,且胃液比肠液更利于促进抗氧化能力的提升.3种银耳酵素经模拟胃肠消化后仍具有较 高的抗氧化活性,其中混合菌种发酵银耳酵素的·OH和DPPH清除率最高,乳酸菌发酵银耳酵素的·O2-清除能力最强,二者的铁离子还原力相近,可为开发具有抗氧化活性的银耳酵素提供科学依据.
Effects of Tremella fuciformis fermentation on antioxidant activity in vitro digestion
To study the effects of in vitro digestion on antioxidant activity of Tremella fuciformis fermentation,three kinds of fermentation by saccharomyces,lactic acid bacteria,and mixed strains used as digestion objects,and·OH,DPPH,O2-clearance rate and iron atomic reducing power used as antioxidant indexes,the changes of their antioxidant capacity during simulated digestion in vitro were analyzed.In the process of in vitro gastrointestinal digestion,the antioxidant activity of the three Tremella fuciformis fermentation was significantly enhanced except that the DPPH scavenging ability of the saccharomyces fermented Tremella fuciformis fermentation was reduced in the intestinal digestion stage,and the gastric fluid was more conducive to promoting the antioxidant capacity than the intestinal fluid.The three Tremella fuciformis fermentation still showed high antioxidant activity after simulated digestion environment,among which the·OH and DPPH scavenging rates of the mixed strains were the highest,and the·O2-scavenging ability of the lactic acid bacteria was the strongest.The iron reducing power of the two kinds of fermentation was similar,which provides a scientific basis for the development of Tremella fuciformis fermentation with antioxidant activity.

Tremella fuciformisfermentationin vitro digestionantioxidant activity

孙嘉蔚、张恩泽、代瀚哲、单振广、李强、张平平

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天津农学院 食品科学与生物工程学院,天津 300392

承德大金星食品有限公司,河北 滦平 068253

天津齐心食品集团有限公司,天津 301599

银耳 酵素 体外消化 抗氧化活性

横向项目

TNHXKJ2023019

2024

天津农学院学报
天津农学院

天津农学院学报

影响因子:0.612
ISSN:1008-5394
年,卷(期):2024.31(4)
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