首页|2种肉桂醛制剂对水产动物致病菌抑菌、杀菌效果比较

2种肉桂醛制剂对水产动物致病菌抑菌、杀菌效果比较

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肉桂醛具有良好的抑菌、杀菌效果,但难溶于水、易氧化的特性限制了肉桂醛的应用范围.为提高肉桂醛水溶性并避免被氧化,本研究构建了纳米乳和羟丙基(β)环糊精包合物两种肉桂醛制剂,以链霉素为阳性对照,比较二者对创伤弧菌(Vibrio vulnificus)、哈维弧菌(Vibrio harveyi)、荧光假单胞菌(Pseudomonas fluorescens)、嗜水气单胞菌(Aeromonas hydrophila)、迟缓爱德华氏菌(Edwardsiella tarda)、温和气单胞菌(Aeromonas sobria)的抑菌、杀菌效果.结果表明:肉桂醛纳米乳制剂对上述 6 种水产致病菌的最小抑菌浓度(MIC)为 0.93、2.05、1.67、14.88、9.30、1.86 mg/mL,最小杀菌浓度(MBC)为 2.40、6.00、1.86、16.00、10.23、5.00 mg/mL,抑杀效果均大幅度优于肉桂醛-羟丙基(β)环糊精包合物.结论:与羟丙基(β)环糊精包合物相比,肉桂醛纳米乳制剂有更高的载药量,对致病菌有更强的抑菌、杀菌活力.虽然两种制剂对致病菌的抑菌、杀菌活力远低于链霉素,但是在绿色无抗水产养殖中仍具有潜在的应用前景.
Comparison of antibacterial and bactericidal effects of two cinnamaldehyde preparations on aquatic animal pathogens
Cinnamaldehyde has good antibacterial,sterilization effect,but the features of being poorly soluble in water and easy oxidation limit its application range.In this study,cinnamaldehyde loaded nano-emulsion and hydroxypropyl(β)cyclodextrin inclusion were prepared.The determination of their antibacterial effect on 6 kinds of pathogenic bacteria-Vibrio vulnificus,Vibrio harveyi,Pseudomonas fluorescens,Aeromonas hydrophila,Edwardsiella tarda and Aeromonas sobria was applied with streptomycin as positive control.The results showed that the minimum inhibitory concentration(MIC)of cinnamaldehyde nano-emulsion against the six aquatic pathogenic bacteria mentioned above were 0.93,2.05,1.67,14.88,9.30,and 1.86 mg/mL,respectively.The minimum bactericidal concentration(MBC)were 2.40,6.00,1.86,16.00,10.23 and 5.00 mg/mL,respectively.The antibacterial effect of cinnamaldehyde nano-emulsion was significantly better than that of cinnamaldehyde hydroxypropyl(β)cyclodextrin inclusion.Conclusion:compared with cinnamaldehyde hydroxypropyl(β)cyclodextrin inclusion,cinnamaldehyde nano-emulsion has higher drug loading rate and better antibacterial activity against pathogenic bacteria.Although the antibacterial and bactericidal activity of the two formulations against pathogenic bacteria is much lower than that of streptomycin,they still have potential application prospects in aquaculture.

cinnamaldehydenano emulsionminimum inhibitory concentrationminimum bactericidal concentrationaquatic pathogenic bacteria

韩鑫怡、王庆奎、姚雨馨、韩妞妞、刘黎瑶

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天津农学院 水产学院,天津 300392

天津市水产生态及养殖重点实验室,天津 300392

天津农学院 基础科学学院,天津 300392

肉桂醛 纳米乳 最小杀菌浓度 最小抑菌浓度 水产致病菌

国家级大学生创新创业项目国家重点研发计划现代农业产业技术体系专项天津市海水养殖产业技术体系创新团队天津市绿色生态饲料重点实验室开放基金天津市水产生态及养殖重点实验室平台项目水产生态与养殖国家级实验教学示范中心(天津农学院)

2019100610032020YFD0900600CARS-47ITTMRS2021000TJ202310

2024

天津农学院学报
天津农学院

天津农学院学报

影响因子:0.612
ISSN:1008-5394
年,卷(期):2024.31(5)