Effects of glutathione treatment on the metabolism of glucosinolates in broccoli seedlings
In order to investigate the effects of exogenous glutathione(GSH)on the metabolism of glucosinolates,brocolli seedlings treated with 25 mg/L GSH were taken as experiment materials.The contents of glucosinolates and sulforaphane and the activity of myrosinase were detected,and the transcriptional expression characteristics of the genes related to glucosinolate metabolism in brocolli seedlings,including bolMAMl,bolUGT74B1,bolMYB28,bolMyr,and bolPEN2 were evaluated at different time points after GSH treatment.The results showed that at 12 h of exogenous GSH treatment,the content of glucosinolate in brocolli seedlings was significantly higher than that in the control.Consistent with this,the transcriptional expression levels of key genes for glucosinolates synthesis,bolMAM1 and bolUGT74B1 were significantly higher than those in the control.Although the transcriptional expression level of bolMYB28 was higher than that in the control,it did not reach a significant level.Further analysis revealed that the expression levels of the myrosinase genes bolMyr and bolPEN2,as well as the myrosinase activity,reached their maximum at 24 h of exogenous GSH treatment and were significantly higher than those in the control.With the increase of myrosinase activity,after 36 h of exogenous GSH treatment,the content of sulforaphane in the treatment group reached its highest value and was significantly higher than that in the control group.In summary,exogenous application of GSH has a positive effect on the synthesis of glucosinolates and the generation of the bioactive substance sulforaphane in brocolli seedlings.The research results provide important data support for the use of exogenous treatment methods to increase the content of glucosinolates,especially its hydrolysis product sulforaphane,in cruciferous plants such as broccoli.
万方数据
维普
