摘要
为提高猪胰脂肪酶的催化作用,采用物理吸附的方法,在柱形玻璃瓶中,在37℃、160 r/min条件下,将固定在小麦麸皮上,将其用于在37℃、160 r/min条件下,催化正己醇与乙酸乙烯酯发生非水转酯反应及花生油水解反应.结果发现,当麸皮与酶的质量比为20:10(mg:mg)时,固定化酶的非水催化活性最高,相对于10 mg的酶粉,活性提高10倍以上.在循环催化转酯反应过程中,在第5次使用之后,固定化酶催化底物反应的转化率降低不足0.2％,显示固定化酶具有较好的催化稳定性.在催化花生油水解的过程中,固定化酶水解花生油的能力相当于酶粉的4.7倍,显示其有用作饲料添加剂的潜力,以辅助动物消化.
Abstract
To enhance the catalytic ability of porcine pancreas lipase,it was immobilized on wheat bran by physical adsorption in a column glass bottle at 37 ℃ and 160 r/min,and used to catalyze the non-aqueous transesterification between hexanol and vinyl acetate as well as the hydrolysis of peanut oil at 37 ℃ and 160 r/min.It was found that the suitable mass ratio of wheat bran and lipase was determined to be 20∶10(mg:mg).At this ratio,the immobilized lipase behaved the highest non-aqueous activity,being more than10-fold activity of 10 mg native lipase.The activity decreased less than 0.2％after the immobilized lipase was reused 5 times in the organic system,each time for 8 h,which showed that it had a good catalytic stability.In hydrolysis of peanut oil,the immobilized lipase could hydrolyze peanut oil 4.7 folds of that accomplished by the native lipase with equal mass,which means that it has potential as a feed additive to assist animal digestion.
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