Screening of Liquid Medium and Fermentation Conditions for Lentinus edodes 0912 by Shaking Flask
The utilization efficiency of nutrients in liquid medium was different among different strains.In the study, Lentinus edodes0912 was used as the tested strain.The optimal carbon and nitrogen sources, inorganic salt content, rotational speed and initial pH of culture medium, and also the standing time and medium volume were screened through the single factor experiment, and the dry weight of mycelia was as the evaluation index.The results showed that the best carbon source was glucose, the best nitrogen source was corn pulp, the best amount of K2HPO4 and KH2PO4 was both 0.1％, the initial pH was 5.0, the liquid medium volume was 50 mL, the culture temperature was 25 ℃, the rest time was 24 h and the speed was 180 r·min-1.On the basis, the composition of liquid medium was tested by L9 (34) orthogonal test.The results showed that the effects of four factors on the dry weight of L.edodes were glucose>corn pulp> KH2PO4>K2HPO4, in which the content of glucose and corn pulp had a significant effect on the dry weight of mycelium (P<0.01) , and the optimum conditions for liquid deep fermentation of L.edodes strain 0912 were glucose 3％, corn pulp 0.3％, K2HPO40.05％, KH2PO40.05％, matched with MgSO40.05％ and VB10.005％, the dry weight of mycelium could reach 2.103 g·L-1, which was 1.49 times of that before optimization (1.417 g·L-1).
L. edodesliquid strainculture medium screeningfermentation by shaking flask
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