The Identification of Spring Wheat Germplasm for End-use Quality and Sequence Analysis of High Molecular Weight Glutenin Subunits
Enhancing the end-use quality of wheat is a major goal in wheat breeding since wheat is an essential food crop.This study examined three accessions,SG-1,SG-2,and SG-3,which showed excellent agronomic traits among the collected germplasm re-sources,in order to improve the genetic resources for wheat end-use quality.The study investigated their the protein content of seeds stored for storage,end-use quality,ways of utilization and breeding values,end-used quality,and SDS-PAGE,A-PAGE,gene se-quencing of genes encoding high molecular weight glutenin subunits,and SDS sedimentation value analysis were used to examine the composition of seed storage protein subunits in different wheat varieties.The results revealed that SG-1 carried the high molecular weight glutenin subunit Dx2+Dy12,whereas SG-2 and SG-3 carried the high molecular weight glutenin subunit Dx5+Dy10.In terms of gliadin,Gli-γ1 was found in all three varieties,however only Gli-γ2 was found in SG-3.The end-use of quality of SG-1 was found to be superior to that of Jinqiang 6,a wheat variety with good end-use quality.Sequence analysis of high molecular weight glutenin subunits identified variation in the By8 subunit of SG-1 and SG-3,indicating that although they shared identical subunits,there was still variation in the sequence.This variation in sequence could potentially explain the poorer performance in end-use quali-ty observed in varieties carrying elite high-quality subunits.Interestingly,the researchers also noted that SG-1,despite carrying a non-premium subunit,exhibited elite end-use quality.This suggests the existence of alternate pathways by which SG-1 can improve wheat end-use quality.Overall,this study expands the genetic resources accessible for wheat end-use quality breeding while also providing new resources and a theoretical basis for studying the mechanism of wheat end-use quality improvement.
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