The Study on Efficient Rapid Propagation System of Lavender
The purpose of this study was to screen out the optimal culture conditions for rapid propagation of lavender by plant tissue culture technology.The seeds of two lavender varieties French Blue and Space Bluewere used as explants to study and analyze the dis-infection methods of explants,the optimal medium and the ratio of hormones for proliferation,differentiation and rooting culture.The results showed that the treatment methods with the highest germination rate and the lowest contamination rate of French Blue and Space Blue seeds were 10%NaClO 15-20 min and 10%H2O25 min,respectively.When the gibberellin(GA3)concentration was 600 mg·L-1,the germination rate of French Blue and Space Bluewas the highest after soaking seed for 16 h and 24 h,respectively.The best proliferation and differentiation medium ofFrench Blue was MS+0.1 mg·L-1 NAA+0.2 mg·L-1 TDZ,and MS+0.05 mg·L-1 NAA+0.2 mg·L-1 TDZ was the optimal proliferation and differentiation medium for Space Blue.The best rooting medium of French Blue and Space Blue was 1/2MS+0.2 mg·L-1 NAA.In this study,a rapid propagation system of lavender was established,which could provid a technical foundation for the rapid propagation,culture expanding,popularizing and application of fine germplasms of lavender.
lavendergibberellin(GA3)proliferation and differentiationrootingrapid reproduction
万方数据
维普
