Kinship Analysis of Hemerocallis fulvay Plants Based on EST-SSR Molecular Markers
Hemerocallis fulvay is a perennial hosta with great ornamental value and has a long history of cultivation in China.In order to explore the kinship relationship among 36 Hemerocallis fulvay species.In this study,EST-SSR molecular marker detection of 36 Hemerocallis fulvay species was carried out by capillary electrophoresis fluorescence labelling technique,and PCR amplification of 36 test Hemerocallis fulvay materials was carried out using 11 pairs of primers with good polymorphism.The results showed that a total of 40 polymorphic loci were obtained from the 11 primer pairs,with an average number of 2.58 effective alleles in the samples,an aver-age Shannon diversity index of 1.00,an average expected heterozygosity of 0.57,and an average observed heterozygosity of 0.52.Analysed by the genetic distance matrix,the genetic distances among the 36 Hemerocallis fulvay varieties ranged from 0.05 to 0.70,the clustering results by UPGMA method using NTSYS software showed that the 36 Hemerocallis fulvay varieties were classified into two major classes when the genetic distance of 0.48 was the threshold,and the first major class could be further divided into four sub-classes when the genetic distance of 0.414 was the threshold.In conclusion,the 36 Hemerocallis fulvay varieties for testing have high genetic diversity and richer genetic backgrounds,which provides a certain theoretical basis for cultivating excellent new varieties of Hemerocallis fulvay in the future.
万方数据
维普
