The Screening and Effect Evaluation of Probiotics for Breeding
In order to obtain excellent strains for preparation of probiotics and meet the needs of feeding probiotics at different stages,a simple and effective index evaluation system for probiotics was established.Twenty strains of probiotics from different sources were compared and screened in terms of acid production,antibacterial effect,hemolysis,antibiotic sensitivity,bile salt tolerance,high tem-perature tolerance and survival ability in simulated gastric juices.The results showed that the probiotic properties of 7 kinds of probi-otics in vitro were consistent with those of feed probiotics.In which,the Bacillus subtilis CICC10089 showed good acid-producing and bacteriostatic effects,ZWRB and Lactobacillus casei-P had strong antibacterial ability.SL and ZW had weak acid production capaci-ty,ZP and NG2 had strong acid production capacity.From the experimen,the evaluation methods of feed probiotics and evaluation indicators for feed probiotics wer established,which could provide reference for the subsequent industrial production of feeding probiotics.