摘要
为查清山西省吕梁市中阳县某猪场从山东购买的保育仔猪大量出现的急性死亡、关节发炎、呼吸困难、高热等症状的病原,无菌采集病猪并带回实验室进行临床剖检,随后采集血液、淋巴结、肺、肾脏和脾脏进行实验室诊断,做出初步判断,制定综合防治策略,病情得到控制.本研究采用灵敏度和准确度较高的实时荧光PCR技术定性检测病料样品中的猪繁殖与呼吸综合征病毒(PRRSV)、非洲猪瘟病毒(ASFV)、猪圆环病毒2型(PCV2)、猪瘟病毒(CSFV)、猪伪狂犬病毒(PRV)和副猪嗜血杆菌(HPS).结果表明:中阳县某猪场保育仔猪为PCV2与HPS混合感染.基于诊断结论对保育猪免疫治疗程序和消毒进行合理调整,综合防治13 d后,猪群无新增发病率和新增死亡率,最大程度地减少了经济损失.本研究为猪场后续的治疗与预防提供了诊断依据.
Abstract
In order to identify the pathogens causing acute death,arthritis,breathing difficulties,and high fever in a large number of piglets purchased from Shandong by a pig farm in Zhongyang County,Lyuliang City,Shanxi Province,this study collected diseased pigs aseptically and brought them back to the laboratory for clinical dissection.Subsequently,blood,lymph nodes,lungs,kidneys,and spleen were collected for laboratory diagnosis,and a preliminary judgment was made.A sensitive and accurate technique,Real time PCR,was used to qualitatively detect porcine reproductive and respiratory syndrome virus(PRRSV),African swine fever virus(ASFV),porcine circovirus type 2(PCV2),swine fever virus(CSFV),porcine pseudorabies virus(PRV),and hemophilusparasuis(HPS)in dis-eased samples.The results showed that PCV2 and HPS mixed infection was confirmed.Based on diagnostic conclusions,adjust the im-munization program of the pig farm to maximize the economic losses caused by the occurrence of diseases.The research results pro-vide diagnostic basis for the subsequent treatment and prevention of pig farms.
基金项目
山西农业大学"科技创新提升工程"项目(CXGC2023018)
中央引导地方科技发展资金项目(YDZJSX2022C023)
山西省重点研发计划项目(201903D221005)
国家科技期刊平台
NSTL
万方数据