Effect of AMEP Protein on Resistance to Low Temperature Chilling Stress in Maize Seedling Stage
To explore the effect of AMEP protein on the physiological metabolism of maize seedlings under low-temperature stress,maize seedlings at two-leaf and one-heart stage were sprayed with 0.1 mg·mL-1 AMEP protein.After 24 hours,the seedlings were treated with low-temperature stress at 0-4℃for 6 hours.Then the morphological parameter,aboveground and belowground biomass,membrane damage indexes,osmoregulation substance,and antioxidant enzyme activities were measured 24 hours after the stress was removed.The results showed that the morphological parameter and organic matter accumulation increased significantly.Compared with the control group,the plant height and root length increased by 23.7%and 29.5%.The dry weight and fresh weight of the above-ground parts increased by 39.3%and 53.2%,and the dry weight and fresh weight of the belowground parts increased by 64.4%and 63.9%.The damage to cell membranes caused by low-temperature stress was significantly reduced,with superoxide anion content,hydrogen peroxide content,malondialdehyde content,and relative conductivity decreased by 36.5%,23.4%,43.8%,and 26.1%,re-spectively.The intracellular osmotic pressure was balanced,while the activity of antioxidant enzymes was also enhanced.The contents of soluble sugar,soluble protein and proline increased by 25.2%,46.1%,26.4%.The activities of SOD,POD,CAT,and APX in-creased by 28.6%,8.3%,22.8%and 28.7%,respectively.In conclusion,AMEP protein can effectively enhance the stress resistance of maize seedlings,thereby reducing the damage caused by low-temperature stress.
AMEP proteinmaizelow temperature damagestress resistance
万方数据
维普
