牛尾山药茎尖培养及快繁技术研究

Research on Shoot Tip Culture and Rapid Propagation Technology of Niuwei Yam

程园 ¹徐升胜 ¹苏樱楠 ¹王仕玉 ¹郭凤根 ²龙雯虹³

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作者信息

1. 云南农业大学园林园艺学院,云南昆明 650201
2. 云南农业大学农学与生物技术学院,云南昆明 650201
3. 云南农业大学园林园艺学院,云南昆明 650201;云南省蔬菜生物学重点实验室,云南昆明 650201
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摘要

为建立牛尾山药脱毒培养和快繁体系,采用培养技术,筛选茎尖培培养、茎段扩繁和生根培养适宜的培养基,并研究适宜的炼苗条件.结果表明:在MS+0.5 mg·L-16-BA+1.5 mg·L-1NAA培养基中,茎尖的膨大率和成活率较高;对茎尖的褐变现象抑制效果最佳的聚乙烯吡咯烷酮(PVP)浓度为 0.01%,其褐变率仅为 11.67%;培养基MS+1 mg·L-16-BA+0.3 mg·L-1NAA诱导节茎段萌芽的效果较好;在生根培养中,MS+0.5 mg·L-16BA+1 mg·L-1NAA培养基最合适;混合基质(珍珠岩∶秸秆腐殖物=1∶1)、温度20℃、遮光率 75%、相对湿度 80%是山药组培苗最适宜的炼苗条件.综上,本研究为牛尾山药脱毒培养和快繁奠定基础,对山药产业可持续发展有重要意义.

Abstract

In order to establish a virus-free culture and rapid propagation system for Niuwei yam,suitable culture media for shoot tip culture,stem segment propagation,and rooting culture were screened using tissue culture techniques,and the appropriate conditions of acclimatizating plantlets were studied.The results showed that the expansion rate and survival rate of shoot tips were higher in the medium MS+0.5 mg·L-16-BA+1.5 mg·L-1NAA.The optimal concentration of polyvinylpyrrolidone(PVP)for inhibiting shoot tip browning was 0.01%,with a browning rate of only 11.67%.The medium MS+1 mg·L-16-BA+0.3 mg·L-1NAA was better for adven-titious bud emergence from a stem segment with a node.In the rooting culture,the medium MS+0.5 mg·L-16BA+1 mg·L-1NAA was the most appropriate.The optimal acclimatization conditions for yam plantlets were mixed substrate(perlite:straw humus=1:1),temper-ature of 20℃,shading rate of 75%,and relative humidity of 80%.In summary,this study lays the foundation for virus-free culture and propagation of Niuwei yam,which is of great significance for the sustainable development of yam industry.

关键词

山药/茎尖培养/茎段培养/褐变抑制/炼苗

Key words

yam/shoot tip culture/shoot segment culture/browning inhibition/acclimatization

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出版年

2024

天津农业科学

天津农业科学院信息研究所

天津农业科学

影响因子：0.705

ISSN：1006-6500

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