摘要
为深入探究藻类多糖对作物种子萌发及幼苗生长的影响,以小麦品种济麦22为材料,设置不同浓度(0、0.25、0.50、1.00、2.00、4.00、8.00 mg·L-1)的普通念珠藻多糖处理组,测定萌发、形态、生理指标.结果表明:随着藻类多糖浓度的提高,小麦种子的萌发指数及幼苗的生长指数呈现低促高抑的趋势.与CK相比,T1、T2、T3和T4处理不同程度地增加了小麦种子的发芽指数,促进了幼苗根叶的生长,增加了幼苗叶绿素、可溶性糖和可溶性蛋白的含量,T5和T6处理不同程度地降低了这些指标.T3处理下,这些指标达到最大值,与CK相比,发芽率、发芽势、发芽指数分别增加16.3％、10.0％和10.3％,根叶长分别增加2.48、1.90 cm,叶绿素、可溶性糖和可溶性蛋白分别增加68.04％、35.20％和42.02％.主成分分析结果表明,第一和第二主成分贡献率分别为81.8％和9.1％,二者贡献率之和为90.9％,指标间的相关性显著,并且种子萌发参数受到根长的影响.综上,施加适量浓度的藻类多糖能显著提高小麦种子的萌发率,促进小麦幼苗的生长,本试验条件下,施加浓度为1 mg·L-1的藻类多糖时,小麦种子的萌发率最高,幼苗生长的效果最佳.该研究结果确定了藻类多糖对小麦的最佳施用浓度,为深入研究其在农业领域的潜在应用提供理论依据,也为藻类多糖在植物生产中的科学施用与广泛推广奠定了基础.
Abstract
To investigate the effects of algal polysaccharides on seed germination and seedling growth,this study used wheat variety Ji Mai 22 as the material and applied varying concentrations(0,0.25,0.50,1.00,2.00,4.00,8.00 mg·L-1)of Nostoc commune polysac-charide.The study assessed the germination,morphological,and physiological indexes of the seedlings.The results revealed that as the concentration of algal polysaccharides increased,the wheat seed germination index and seedling growth index followed a pattern of initial low promotion,which was subsequently followed by strong inhibition.Compared to the control group(CK),treatments T1,T2,T3,and T4 enhanced the wheat seed germination index,promoted root and leaf growth,and increased chlorophyll,soluble sugar,and soluble protein levels in the seedlings.In contrast,treatments T5 and T6 resulted in a decrease in these parameters.The highest val-ues were observed under the T3 treatment.Specifically,compared to CK,the germination rate,germination potential,and germination index increased by 16.3％,10.0％,and 10.3％,respectively.Additionally,root and leaf lengths increased by 2.48 cm and 1.90 cm,respectively,while chlorophyll,soluble sugar,and soluble protein content rose by 68.04％,35.20％,and 42.02％,respectively.Princi-pal component analysis indicated that the first and second principal components accounted for 81.8％and 9.1％of the total variance,respectively,with the combined contribution of the two components reaching 90.9％.Significant correlations were found between the indexes,and seed germination was particularly influenced by root length.In conclusion,the application of an optimal concentration of algal polysaccharide can significantly enhance the germination rate and promote seedling growth in wheat.Under the experimental conditions,the highest germination rate and best seedling growth were achieved with a concentration of 1 mg·L-1 algal polysaccharide.These findings contribute to identifying the optimal application concentration of algal polysaccharides,provide a theoretical foundation for further research into their agricultural applications,and support the scientific use and potential widespread adoption of algal polysaccharides in plant cultivation.
维普
万方数据