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甲咪唑烟酸降解细菌的筛选与鉴定

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为实现环境污染物的生物治理与资源化利用,以花生连作田土壤中的甲咪唑烟酸降解细菌为研究对象,采用水解圈法和降解能力测定进行筛选,对筛选出的菌株进行形态学、生理生化特性和分子生物学鉴定.结果表明:本研究共筛选出8株甲咪唑烟酸降解细菌,分别为大山芽孢杆菌(Baeillus gaemokensis)、苏云金芽孢杆菌(Bacillus thuringiensis)、蜡样芽孢杆菌(Bacillus cereus)、同温层芽孢杆菌(Bacillus stratosphericus)、甲基营养型芽孢杆菌(Bacillus methylotrophic us)、侧孢短芽孢杆菌(Brevibacil-lus laierosporus)和恶臭假单胞(Pseudomomas puida).其中,芽孢杆菌属占了 7株,为所筛选的甲咪唑烟酸降解菌中的优势细菌.大山芽孢杆菌的降解能力最强,降解率达到88.23%.综上,这些菌株为研究微生物降解甲咪唑烟酸提供了菌种资源,为未来开发甲咪唑烟酸微生物降解制剂和修复土壤提供了理论基础.
Screening and Identification of Imazapic Degrading Bacteria
To achieve the biological treatment and resource utilization of environmental pollutants,the imazapic degrading bacteria from peanut continuous cropping soil were selected by hydrolytic circle method and degradation ability measurement.The strains were iden-tified in terms of morphology,physiological and biochemical characteristics and molecular biology.The results showed that 8 imazapic degrading strains were selected,which were identified as Bacillu gaemokensis,Bacillus thuringiensis,Bacillus cereus,Bacillus strato-sphericus,Bacillus methylotrophic,Brevibacillus laterosporus and Pseudomonas putidis.The dominant strain of bacteria responsible for imazapic degradation was Bacillus sp..The degradation ability of B.gaemokensis was the strongest,which the degradation rate reached 88.23%.In conclusion,the aforementioned strains have established a theoretical framework for the study of microbial degradation of i-mazapic,as well as for developing microbial degradation agents and soil remediation strategies.

imazapicdegrading bacteriaBacillus sp.screeningidentification

唐琳、王子妍、陈雪冬、刘佳宜

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洛阳师范学院生命科学学院,河南洛阳 471934

甲咪唑烟酸 降解细菌 芽孢杆菌属 筛选 鉴定

2024

天津农业科学
天津农业科学院信息研究所

天津农业科学

影响因子:0.705
ISSN:1006-6500
年,卷(期):2024.30(11)