Construction and Properties of Catalytic Hybrid Nanobiocatalyst CALB@Fe3O4@ZIF-8
Lipase(CALB)was used as the model enzyme.When functional nanomaterial ferric oxide nanoparticles were hybridized with metal-organic framework(MOF)material ZIF-8,CALB was embedded in the hybrid material at the same time to prepare hybrid nanobiocatalysts CALB@Fe3O4@ZIF-8.The effects of temperature,mechanical stirring speed,illumination and the amount of Fe3O4 on the activity of CALB@Fe3O4@ZIF-8 catalyst were investigated.The optimum con-ditions for preparing CALB@Fe3O4@ZIF-8 were as follows:temperature 20℃,rotation speed 400 r/min,illumination wavelength 380 nm and Fe3O4 mass 5 mg.Under these conditions,the maximum recovery rate of CALB@Fe3O4@ZIF-8 was 82%,which was 16%higher than before optimization.At the same time,with the addition of Fe3O4 nanoparticles with pho-tothermal effect,CALB@Fe3O4@ZIF-8 showed strong photothermal effect,light intensity and wavelength could signifi-cantly improve the affinity and reaction rate of enzyme and substrate,and CALB@Fe3O4@ZIF-8 showed higher apparent enzyme activity.This study provides a useful reference for the development of novel functional nanohybrid MOF immo-bilized enzyme biocatalysts.
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