Expression and Characterization of Novel Sulfotransferases in Escherichia coli
Heparin is a highly sulfated and heterogeneous linear glycosaminoglycan of animal origin.Aryl sulfotransferase participates in the synthesis and regeneration of 3′-pho-sphoadenosine-5′-phosphosulfate(PAPS)of the sulfonic acid donor in heparin biosynthesis,and is a key enzyme to realize the physiological function of heparin.At present,aryl sulfotrans-ferases have been reported to have such problems as few species and low expression amount.In our present study,the hidden Markov model was used to obtain 10 sulfotransferases derived from birds,and the codon was optimized and fully synthe-sized into the expression vector pET-32a,and the E.coli BL21 was transferred to induce expression.The heterologous ex-pressions of S4 and S8 were achieved successfully by optimizing the medium composition,induction temperature,isopropylthio-β-D-galactoside(IPTG)concentration and shaker speed.In TB medium,induction at 16℃using 0.4 mmol/L IPTG was expressed with strain BL21/pET32a-Mbp-S4 at 200 r/min.The expression of the target protein reached 60.6 mg/L.The purification and characterization of S4 proteins showed that the optimal reaction temperature,pH and specific enzyme activity reached 40℃,7.0 and 3.3 mU/mg,respectively.The modified product was detected by UPLC-MS,and S4 was fur-ther verified as sulfonyltransferase.The successful expression of novel sulfotransferases S4 has provided an effective element for PAPS regeneration.
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