Characteristics and molecular ecological network analysis of nitrogen cycling microbial communities at farmland unsaturated zone in Tianjin
Accumulation of nitrate in unsaturated zone of soil can cause groundwater nitrogen pollution,which demonstrates serious impact on ecology and human health.To investigate the relationship between nitrate reduction and microbial communi-ties in unsaturated zone,soil physico-chemical properties were measured,and 16S rRNA high-throughput technology and molecular ecological network were used to analyze the structure and characteristics of the nitrogen cycling microbial communi-ties in the unsaturated zone,where soil samples(0-3 m)were collected from farmland in Tianjin.The results showed that Thau-marchaeota and Proteobacteria were the dominant phylum,and microbial communities were significantly correlated with pH value,ammonium content and nitrite content.Ammonia oxidizing archaea,ammonia oxidizing bacteria,nitrite oxidizing bac-teria,denitrifying bacteria,anaerobic ammonium oxidation bacteria,dissimilatory nitrate reduction to ammonium bacteria,and NC10 phylum bacteria appeared in the molecular ecological network of soil microbial communities for nitrogen cycling.Denitrifying bacteria are the main driver of nitrate reduction.The competitive relationship between denitrifying bacteria and ammonia oxidizing archaea,nitrite oxidizing bacteria and dissimilatory nitrate reduction to ammonium bacterial communities maintained the microbial communities stable,while the cooperative relationship between the communities promoted the nitro-gen cycle,thus ensures the sustainability of nitrate reduction.
unsaturated zonenitrogen cyclemicrobial community characteristicsmicrobial molecular ecological network
万方数据
维普
